Estranediols profiling in calves' urine after 17beta-nandrolone laureate ester administration
| Autor: | Fabrice Monteau, Bruno Le Bizec, Lauriane Rambaud, Gaud Pinel, Christopher Elliot |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Urinary system medicine.medical_treatment Metabolite Estranes Clinical Biochemistry Urine Biochemistry Gas Chromatography-Mass Spectrometry Steroid chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Nandrolone Molecular Biology Chromatography Chemistry Esters Cell Biology Metabolism Molecular Medicine Cattle Gas chromatography–mass spectrometry Anabolic steroid medicine.drug |
| Zdroj: | The Journal of steroid biochemistry and molecular biology. 121(3-5) |
| ISSN: | 1879-1220 |
| Popis: | 17beta-Nandrolone (17beta-NT) is one of the most recurrent forbidden anabolic steroid used in meat producing animals breeding. Because efficient control must both take into account metabolic patterns and associated kinetics of elimination, the metabolism of 17beta-NT in bovines has already been investigated and is well documented, but only focussing on its main metabolites (i.e. 17alpha-nandrolone, 19-noretiocholanolone and 19-norandrostenedione). The goal of the present study was to enlarge this panel of 17beta-NT metabolites, especially through the urinary estranediols fraction in order to perform a more global steroid profiling upon 17beta-nortestosterone laureate ester administration in calves. A GC-MS/MS method has been developed to monitor and quantify 5 estranediols isomers including 5alpha-estrane-3beta,17beta-diol (abb), 5beta-estrane-3alpha,17beta-diol (bab), 5alpha-estrane-3beta,17alpha-diol (aba), 5alpha-estrane-3alpha,17beta-diol (aab) and 5beta-estrane-3alpha,17alpha-diol (baa). Their urinary elimination kinetics have been established allowing detection of 4 estranediols up to several days after administration. All animals demonstrated homogeneous patterns of elimination both from a qualitative (metabolite profile) and quantitative point of view (elimination kinetics in urine). 5alpha-Estrane-3beta,17alpha-diol (aba) was found as the major metabolite with concentrations up to 100microgL(-1). |
| Databáze: | OpenAIRE |
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