Enzymatic synthesis of isoamyl butyrate catalyzed by immobilized lipase on poly-methacrylate particles: optimization, reusability and mass transfer studies

Autor: Flávia Arantes Pires Lage, Larissa Midiane Todero, Daniela B. Hirata, Jaquelinne de Jorge Bassi, Adriano A. Mendes, Maria Carolina Cucatti Corradini, Jayne C. S. Barboza
Rok vydání: 2015
Předmět:
Zdroj: Bioprocess and Biosystems Engineering. 38:1601-1613
ISSN: 1615-7605
1615-7591
DOI: 10.1007/s00449-015-1402-y
Popis: Isoamyl butyrate (banana flavor) was synthe- sized by esterification reaction of isoamyl alcohol and butyric acid in heptane medium. Immobilized Ther- momyces lanuginosus lipase (TLL) prepared via physical adsorption on mesoporous poly-methacrylate particles (PMA) was used as biocatalyst. The factors that affect the esterification reaction were optimized by response surface methodology (RSM). Under optimal experimental condi- tions, maximum ester conversion percentage of 96.1 and 73.6 % was reached after 50 and 90 min, respectively, for esterification reaction performed at equimolar ratio alco- hol:acid at 500 and 2000 mM of each substrate. Under these experimental conditions, the esterification reaction was not controlled by external and intra-particle mass transfer effects. The product (isoamyl butyrate) was con- firmed by proton nuclear magnetic resonance ( 1 HN MR) spectroscopy. Reusability tests showed that the biocatalyst retained around 96 and 31 % of its initial activity after eight successive esterification cycles performed at 500 and 2000 mM, respectively. The application of the biocatalyst prepared showed to be a promising strategy to catalyze flavor ester synthesis in a non-aqueous medium.
Databáze: OpenAIRE
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