Interleukin-8 has antitumor effects in the rat which are not associated with polymorphonuclear leukocyte cytotoxicity
| Autor: | Reisser D, Pascale Lejeune, Patricia Lagadec, Jean-François Jeannin, Ivan Lindley, Nathalie Onier |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cytotoxicity
Immunologic Cancer Research Neutrophils medicine.medical_treatment Immunology Antineoplastic Agents Granulocyte Adenocarcinoma In vivo medicine Immunology and Allergy Cytotoxic T cell Animals Interleukin 8 Cytotoxicity Immunity Cellular business.industry Interleukin-8 In vitro Rats medicine.anatomical_structure Cytokine Oncology Colonic Neoplasms Cancer research Tumor necrosis factor alpha business |
| Zdroj: | Cancer immunology, immunotherapy : CII. 38(3) |
| ISSN: | 0340-7004 |
| Popis: | The antitumor effect of lipopolysaccharides (LPS) has been observed in several experimental models and is likely to be mediated by macrophages. Stimulation of macrophages with LPS results in the release of several cytokines, including tumor necrosis factor, interleukin-1 and neutrophil-activating peptide-1/interleukin-8 (IL-8), which activates polymorphonuclear leukocytes (PMN) in vitro. Since PMN have an antitumor activity, we tested the in vivo effect of IL-8 on the growth of peritoneal carcinomatoses induced by PROb colon cancer cells in syngeneic rats. IL-8 induced a significant regression of tumors measuring 1-5 mm, and a complete regression was observed in 8 out of 40 rats in four independent experiments. IL-8 was not directly cytotoxic in vitro for tumor cells and was effective in vivo in a narrow range of doses. IL-8 had a significant chemotactic effect for peritoneal PMN in both normal and tumor-bearing rats. PMN taken from the peritoneum of tumor-bearing rats during IL-8 treatment had the same cytotoxic activity against PROb tumor cells as PMN from untreated control rats. Microscopic examinations of tumors during the treatment showed poor infiltrating by PMN. We conclude that the antitumor activity of IL-8 in this model is not mediated by PMN cytotoxicity. |
| Databáze: | OpenAIRE |
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