Inducing β-Sheets Formation in Synthetic Spider Silk Fibers by Aqueous Post-Spin Stretching
| Autor: | Randolph V. Lewis, Michael B. Hinman, Jeffery L. Yarger, Gregory P. Holland, Bo An |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Magnetic Resonance Spectroscopy
Polymers and Plastics Molecular Sequence Data Silk Bioengineering Biocompatible Materials Transfection Article Protein Structure Secondary Biomaterials Protein structure Elastic Modulus Tensile Strength Ultimate tensile strength Polymer chemistry Materials Testing Materials Chemistry Escherichia coli Animals Spider silk Amino Acid Sequence Cloning Molecular biology Spidroin Chemistry Nephila clavipes Biomaterial Water Spiders biology.organism_classification Elasticity Recombinant Proteins SILK Biophysics Stress Mechanical Fibroins MASP1 Plasmids |
| Popis: | As a promising biomaterial with numerous potential applications, various types of synthetic spider silk fibers have been produced and studied in an effort to produce man-made fibers with mechanical and physical properties comparable to those of native spider silk. In this study, two recombinant proteins based on Nephila clavipes Major ampullate Spidroin 1 (MaSp1) consensus repeat sequence were expressed and spun into fibers. Mechanical test results showed that fiber spun from the higher molecular weight protein had better overall mechanical properties (70 KD versus 46 KD), whereas postspin stretch treatment in water helped increase fiber tensile strength significantly. Carbon-13 solid-state NMR studies of those fibers further revealed that the postspin stretch in water promoted protein molecule rearrangement and the formation of β-sheets in the polyalanine region of the silk. The rearrangement correlated with improved fiber mechanical properties and indicated that postspin stretch is key to helping the spider silk proteins in the fiber form correct secondary structures, leading to better quality fibers. |
| Databáze: | OpenAIRE |
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