Morphological and molecular characterization of Stigonema jureiensis sp. nov. (Nostocales, Cyanobacteria) from the Atlantic Rainforest, São Paulo, Brazil
| Autor: | Guilherme Scotta Hentschke, Diego Bonaldo Genuário, Haywood Dail Laughinghouse, Célia Leite Sant'Anna, Janaina Rigonato |
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| Přispěvatelé: | Universidade do Porto, Universidade Estadual Paulista (Unesp), Institut de Biologie François Jacob, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), University of Florida / IFAS, Laboratory of Phycology |
| Rok vydání: | 2019 |
| Předmět: |
Nostocales
Microorganism education.field_of_study Polyphasic approach Terrestrial habitats Phylogenetic tree biology Population Biodiversity Zoology Plant Science Rainforest 16S ribosomal RNA biology.organism_classification Morphological analysis Tropical environments Taxonomy (biology) education Taxonomy |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1805-4927 1802-5439 |
| Popis: | Made available in DSpace on 2020-12-12T01:44:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação para a Ciência e a Tecnologia Tropical regions are considered hotspots of biodiversity, and the genus Stigonema has been underexplored in all world, mainly due to the difficulties of culturing. In this study, we sequenced the 16S rRNA gene of a natural Stigonema population from the tropical Atlantic Rainforest (São Paulo, Brazil) and describe S. jureiensis sp. nov. based on phylogenetic and morphological data. For the molecular analysis, the genomic DNA was isolated from aliquots of the population, which were previously manually cleaned under a microscope, using a point–tapered Pasteur pipette to remove other organisms and debris. For morphological analysis, 30 individuals were analyzed and photographed using microscope. Both maximum likelihood and Bayesian phylogenies support S. jureiensis as a new species, and morphological analysis revealed the presence of a special type of mosaic ornate sheaths. Interdisciplinary Centre of Marine and Environmental Research – CIIMAR Universidade do Porto, Av. General Norton de Matos S/N Department of Zoology and Botany Instituto de Biociências Letras e Ciências Exatas (UNESP), R. Cristóvão Colombo, 2265, Jardim Nazareth CEA Centre de Sequençage Genoscope Institut de Biologie François Jacob EMBRAPA Environment Laboratory of Environmental Microbiology Agronomy Department Fort Lauderdale Research and Education Center University of Florida / IFAS, 3205 College Avenue Institute of Botany Laboratory of Phycology, Av. Miguel Stéfano, 3687, Água Funda Department of Zoology and Botany Instituto de Biociências Letras e Ciências Exatas (UNESP), R. Cristóvão Colombo, 2265, Jardim Nazareth FAPESP: 2014/26131–7 CNPq: Multi/04423/2019 Fundação para a Ciência e a Tecnologia: Multi/04423/2019 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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