Acute psychological stress increases paracellular permeability and modulates immune activity in rectal mucosa of healthy volunteers

Autor: Linda Gerdin, Ana M. González‐Castro, Ann‐Charlott Ericson, Mats Persborn, Javier Santos, Susanna A. Walter, Åsa V. Keita, Maria Vicario, Johan D. Söderholm
Přispěvatelé: Institut Català de la Salut, [Gerdin L] Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden. Department of Surgery, Linköping University, Linköping, Sweden. Surgical Clinic of Jönköping County, Jönköping, Sweden. [González-Castro AM] Grup de Recerca d’Immunologia Translacional, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’Aparell Digestiu, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Ericson AC] Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden. Department of Surgery, Linköping University, Linköping, Sweden. [Persborn M] Surgical Clinic of Jönköping County, Jönköping, Sweden. [Santos J] Grup de Recerca de Fisiologia i Fisiopatologia Digestiva, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’Aparell Digestiu, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain. [Walter SA] Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden. Department of Surgery, Linköping University, Linköping, Sweden. Department of Gastroenterology, Linköping University, Linköping, Sweden. [Vicario M] Grup de Recerca d’Immunologia Translacional, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’Aparell Digestiu, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain. Department of Gastrointestinal Health, Nestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, Switzerland, Vall d'Hebron Barcelona Hospital Campus
Rok vydání: 2022
Předmět:
Zdroj: Scientia
ISSN: 2050-6414
2050-6406
DOI: 10.1002/ueg2.12329
Popis: Background Psychological stress and increased permeability are implicated as contributing factors in the initiation and worsening of gastrointestinal diseases. A link between stress and intestinal permeability has been shown in animal models as well as in human small intestine, but stress effects on the human colorectal mucosal barrier has not been reported. Objective To investigate the potential effects of acute psychological stress on colorectal mucosal barrier function and to explore stress-induced molecular events in the rectal mucosa under healthy conditions. Methods Endoscopic biopsies were taken from the rectosigmoid region of healthy volunteers, who had been subjected to dichotomous listening stress and after a control session, respectively. Paracellular and transcellular permeability were assessed in modified Ussing chambers. RNA expression (microarray technology confirmed by quantitative real-time polymerase chain reaction) and biological pathway analysis were used to investigate the local mucosal response to acute stress. Results Dichotomous listening stress induced a subjective and objective stress response, and significantly increased paracellular but not transcellular permeability. We also identified a stress-induced reduction in RNA expression of genes related to immune cell activation and maturation (CR2, CD20, TCLA1, BANK1, CD22, FDCSP), signaling molecules of homing of immune cells to the gut (chemokines: CCL21, CXCL13, and CCL19, and receptors: CCR7, CXCR5), and innate immunity (DUOX2). Eight of the 10 top down-regulated genes are directly involved in B cell activation, signaling and migration. The systemic stress response correlated positively with paracellular permeability and negatively with DUOX2 expression. Conclusion Dichotomous listening stress increases paracellular permeability and modulates immune cell activity in the rectal mucosa. Further studies are warranted to identify the primary mechanisms of stress-mediated reduction of mucosal defensive activity and barrier dysfunction, and their potential implications for gastrointestinal disorders. Funding Agencies|Fondo de Investigacion Sanitaria and CIBERehd, Instituto de Salud Carlos III, Subdireccion General de Investigacion Sanitaria, Ministerio de Economia y Competitividad [PI17/0190, PI19/01643]; Futurum, Region Jonkoping County [12406, 1460]; Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas [CB06/04/0021]; Vetenskapsradet [2014-02537, 2017-02475]; Forskningsradet i Sydostra Sverige [37841]
Databáze: OpenAIRE
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