Enzymatic amplification of platelet-specific messenger RNA using the polymerase chain reaction
| Autor: | C Cretney, G C White nd, Richard H. Aster, Jack Gorski, Peter J. Newman, S Gidwitz |
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| Rok vydání: | 1988 |
| Předmět: |
Blood Platelets
Five-prime cap biology Oligonucleotide Inverse polymerase chain reaction Platelet disorder Gene Amplification Multiple displacement amplification Nucleic Acid Hybridization RNA-dependent RNA polymerase DNA DNA-Directed DNA Polymerase Platelet Membrane Glycoproteins General Medicine Molecular biology Biochemistry RNA polymerase I biology.protein Humans RNA Messenger Cloning Molecular Polymerase Research Article |
| Zdroj: | Journal of Clinical Investigation. 82:739-743 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci113656 |
| Popis: | Human platelets are derived from megakaryocytes as anucleate cells, and thus contain only vestigial amounts of RNA capable of being transcribed into protein. This has greatly hampered efforts to study directly platelet-specific gene products and their associated polymorphisms. In this report, we describe direct amplification, using the polymerase chain reaction, of platelet-derived mRNA in amounts sufficient to permit detailed analysis, such as restriction mapping and nucleotide sequencing. The ability to generate large amounts of cDNA from platelet-specific mRNA sequences should make possible direct molecular characterization of normal platelet proteins, and facilitate the investigation of a wide variety of inherited platelet disorders. |
| Databáze: | OpenAIRE |
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