Porcine circovirus type 2 (PCV2) induces cell proliferation, fusion, and chemokine expression in swine monocytic cells in vitro
| Autor: | Chian-Ren Jeng, Chih-Cheng Chang, Hui-Wen Chang, Jiuan Judy Liu, Yi Chieh Tsai, Victor Fei Pang, Chun-Ming Lin, Shih Hsuan Hsiao, Mi Yuan Chia |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Circovirus
Chemokine multinucleated giant cell 040301 veterinary sciences Swine animal diseases proliferation Inflammation [SDV.BC]Life Sciences [q-bio]/Cellular Biology Monocytes 0403 veterinary science Cell Fusion 03 medical and health sciences [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] medicine Macrophage Animals chemotaxis 030304 developmental biology Cell Proliferation 0303 health sciences Cell fusion General Veterinary biology monocyte-derived macrophage [SDV.BA]Life Sciences [q-bio]/Animal biology Monocyte virus diseases [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology 04 agricultural and veterinary sciences biology.organism_classification Molecular biology [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics Porcine circovirus [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology medicine.anatomical_structure Gene Expression Regulation Giant cell Immunology biology.protein [SDV.IMM]Life Sciences [q-bio]/Immunology [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Original Article medicine.symptom Chemokines porcine circovirus type 2 |
| Zdroj: | Veterinary Research Veterinary Research, BioMed Central, 2010, 41 (5), ⟨10.1051/vetres/2010032⟩ |
| ISSN: | 0928-4249 1297-9716 |
| DOI: | 10.1051/vetres/2010032⟩ |
| Popis: | International audience; Granulomatous lymphadenitis is one of the pathognomonic lesions in post-weaning multisystemic wasting syndrome (PMWS)-affected pigs. This unique lesion has not been reported in direct association with viral infection in pigs. The objective of the present study was to evaluate whether porcine circovirus type 2 (PCV2) alone is able to induce functional modulation in porcine monocytic cells in vitro to elucidate its possible role in the development of granulomatous inflammation. It was found that the proliferation activity of blood monocytes (Mo) and monocyte-derived macrophages (MDM) was significantly enhanced by PCV2. During monocyte-macrophage differentiation, the PCV2 antigen-containing rate and formation of multinucleated giant cells (MGC) were significantly increased in MDM when compared to those in Mo. The MDM-derived MGC displayed a significantly higher PCV2 antigen-containing rate than did the mono-nucleated MDM. Supernatants from PCV2-inoculated MDM at 24 h post-inoculation induced an increased tendency of chemotactic activity for blood Mo. At the same inoculation time period, levels of mRNA expression of the monocytic chemokines, monocyte chemoattractant protein-1 and macrophage inflammatory protein-1, also significantly increased in PCV2-inoculated MDM. The results suggest that PCV2 alone may induce cell proliferation, fusion, and chemokine expression in swine monocytic cells. Thus, PCV2 itself may play a significant role in the induction of granulomatous inflammation in PMWS-affected pigs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|