Modulation and intracellular transport of CD20 and CD21 antigens induced by B1 and B2 monoclonal antibodies in RAJI and JOK-1 cells--an immunofluorescence and immunoelectron microscopy study
| Autor: | Anne Marie Boesen, Stanislaw Pulczynski, Olaf Myhre Jensen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cancer Research
Time Factors medicine.drug_class Immunoelectron microscopy media_common.quotation_subject Fluorescent Antibody Technique chemical and pharmacologic phenomena Biology Immunofluorescence Monoclonal antibody Endocytosis Flow cytometry Cell Line Antigenic Modulation Antigen immune system diseases Antigens CD hemic and lymphatic diseases medicine Fluorescence microscope Humans Internalization media_common B-Lymphocytes medicine.diagnostic_test Antibodies Monoclonal Biological Transport Hematology Antigens CD20 Flow Cytometry Virology Molecular biology Cell Compartmentation Antigens Differentiation B-Lymphocyte Microscopy Electron Oncology Receptors Complement 3d |
| Zdroj: | Leukemia research. 18(7) |
| ISSN: | 0145-2126 |
| Popis: | By fluorescence microscopy (FM), flow cytometry (FCM) and immunoelectron microscopy (IEM) we have shown that B1 and B2 monoclonal antibodies (MoAbs) were able to induce modulation of CD20 and CD21 in RAJI and JOK-1 cell lines. Redistribution and internalization of both antigens (Ags) after binding with MoAbs was readily demonstrated by FM, and by IEM CD20 and CD21 were found to be processed by the pathway of receptor-mediated endocytosis. The rate of intracellular transport varied: CD21 > CD20 and RAJI > JOK-1. Approximately 65 and 55% of CD20 and 60 and 45% of CD21 were cleared from the surface of RAJI and JOK-1 cells, respectively (FCM and IEM). These values, however, clearly exceeded those corresponding to internalization (11, 9, 24 and 16%) indicating shedding of Ag-MoAb complexes. No evidence of recycling was found. The present data support the hypothesis that the kinetics of modulation vary from one Ag to another and probably also reflect the stage of differentiation of the malignant B-cells. The results are discussed in the context of the possible usefulness of B1 and B2 MoAbs in the therapy of B-cell malignancies. |
| Databáze: | OpenAIRE |
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