Conformational flexibility of fatty acid-free bovine serum albumin proteins enables superior antifouling coatings
| Autor: | Joshua A. Jackman, Nam-Joon Cho, Gamaliel Junren Ma, Abdul Rahim Ferhan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
biology Chemistry Nanoparticle Fatty acid 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Biofouling Film coating Chemical engineering Mechanics of Materials Fatty acid free biology.protein TA401-492 Nanobiotechnology General Materials Science Bovine serum albumin Purification methods 0210 nano-technology Materials of engineering and construction. Mechanics of materials |
| Zdroj: | Communications Materials, Vol 1, Iss 1, Pp 1-11 (2020) |
| ISSN: | 2662-4443 |
| Popis: | Bovine serum albumin (BSA) protein is widely used to fabricate antifouling coatings for nanobiotechnology applications. Numerous BSA protein options are commercially available and obtained through different purification methods, however, there is no guidance on which ones are preferable for antifouling coatings. Herein, we investigate the real-time fabrication of antifouling coatings composed of BSA proteins obtained through different purification methods, and report significant differences in ultrathin film coating properties and ability to mitigate serum biofouling and to prevent nanoparticle-induced immune reactions. Mechanistic studies unravel the source of these performance variations, which are related to some BSA proteins containing fatty acid stabilizers while other BSA proteins are fatty acid-free depending on the purification method. Fatty acid-free BSA proteins exhibit greater conformational flexibility and less charge repulsion, which allow them to form more rigidly attached and tightly packed coatings on flat surfaces and nanoparticles that result in superior application performance. Bovine serum albumin proteins are used to fabricate antifouling coatings, but it is unclear which of these give the best coatings. Here, bovine serum albumin proteins from different purification processes are investigated, revealing that fatty acid-free proteins give superior antifouling properties. |
| Databáze: | OpenAIRE |
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