| Autor: |
Bolton, Jessica S., Sidhartha Chaudhury, Sheetij Dutta, Gregory, Scott, Locke, Emily, Pierson, Tony, Bergmann-Leitner, Elke S. |
| Rok vydání: |
2020 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.12151077 |
| Popis: |
Additional file 1: Figure S1. Overview of ECLIA assay platform. (Panel A) Experimental steps for assay setup. Biotinylated antigens are coupled with proprietary U-PLEX linkers in separate tubes. Once coupling is complete, U-PLEX-coupled antigens are combined into a cocktail and the assay plates coated. Each U-PLEX linker can only bind to its respective spot (color coded in Figure); up to 10 antigens can be coated per well (top view plate well). (Panel B) Overview of all steps to complete the assay: antigen-coated wells are incubated with diluted serum or plasma. Antigen-specific antibodies will bind to the antigen and the binding visualized by adding a Sulfo-Tag-labeled secondary antibody and substrate. (Panel C) Data acquisition. Plate is inserted into reader which will deliver an electric pulse that activates the substrate. A high resolution camera measures the luminescence above each spot. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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