Differentiated human stem cells resemble fetal, not adult, β cells
| Autor: | Felicia W. Pagliuca, Douglas A. Melton, Charles W. O'Donnell, Philip diIorio, Francis Deng, Alireza Rezania, David K. Gifford, Sinisa Hrvatin, Jeffrey R. Millman |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Pluripotent Stem Cells KOSR Multidisciplinary Gene Expression Profiling Cellular differentiation Cell Differentiation Biological Sciences Biology Flow Cytometry Microarray Analysis Molecular biology Fetus P19 cell Cancer stem cell Insulin-Secreting Cells Amniotic epithelial cells Humans Stem cell Induced pluripotent stem cell Pancreas Adult stem cell |
| Zdroj: | Proceedings of the National Academy of Sciences. 111:3038-3043 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Human pluripotent stem cells (hPSCs) have the potential to generate any human cell type, and one widely recognized goal is to make pancreatic β cells. To this end, comparisons between differentiated cell types produced in vitro and their in vivo counterparts are essential to validate hPSC-derived cells. Genome-wide transcriptional analysis of sorted insulin-expressing (INS(+)) cells derived from three independent hPSC lines, human fetal pancreata, and adult human islets points to two major conclusions: (i) Different hPSC lines produce highly similar INS(+) cells and (ii) hPSC-derived INS(+) (hPSC-INS(+)) cells more closely resemble human fetal β cells than adult β cells. This study provides a direct comparison of transcriptional programs between pure hPSC-INS(+) cells and true β cells and provides a catalog of genes whose manipulation may convert hPSC-INS(+) cells into functional β cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|