Tetrodonic acid-like substance; A possible precursor of tetrodotoxin
| Autor: | Sekio Kanoh, Kanehisa Hashimoto, Osamu Arakawa, Abdallah E. Ali, Yasuo Shida, Sachio Nishio, Tamao Noguchi, Keisuke Miyazawa |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Size-exclusion chromatography Tetrodotoxin Toxicology Mass spectrometry High-performance liquid chromatography Gas Chromatography-Mass Spectrometry Mass Spectrometry Mice chemistry.chemical_compound Column chromatography Animals Chromatography High Pressure Liquid Chromatography Electrophoresis Cellulose Acetate Hydrogen-Ion Concentration Fast atom bombardment Invertebrates Secondary ion mass spectrometry chemistry Platyhelminths Quinazolines Spectrophotometry Ultraviolet Chromatography Thin Layer Gas chromatography |
| Zdroj: | Toxicon. 29:845-855 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/0041-0101(91)90221-c |
| Popis: | A tetrodonic acid-like substance which was hardly distinguishable from authentic tetrodonic acid in thin-layer chromatography, high performance liquid chromatography, etc., was successfully purified from the ribbon worm and flatworm by a method consisting mainly of Bio-Gel P-2 column chromatography. The tetrodonic acid-like substance showed a specific toxicity of approximately 700 mouse units/mg as tetrodotoxin, unlike tetrodonic acid which is a completely nontoxic substance. Instrumental analyses including gas chromatography-mass spectrometry, secondary ion mass spectrometry and thin-layer chromatography/fast atom bombardment mass spectrometry disclosed that the tetrodonic acid-like substance had a structure similar to, but not the same as, that of tetrodotoxin. This, along with its high convertibility into tetrodotoxin during storage and other experimental operations, suggested that the tetrodonic acid-like substance is a precursor of tetrodotoxin. |
| Databáze: | OpenAIRE |
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