Protein imprinted materials designed with charged binding sites on screen-printed electrode for microseminoprotein-beta determination in biological samples
| Autor: | Tânia S.C.R. Rebelo, M. Goreti F. Sales, Carlos M. Pereira, João Paulo Noronha, Fernando Silva |
|---|---|
| Přispěvatelé: | Repositório Científico do Instituto Politécnico do Porto |
| Rok vydání: | 2016 |
| Předmět: |
Serum
Calibration curve Screen-printed electrodes 02 engineering and technology Urine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Materials Chemistry MSMB Electrical and Electronic Engineering Binding site Instrumentation Caffeic acid Detection limit Chromatography Metals and Alloys Electrochemical biosensor Protein imprinted materials 021001 nanoscience & nanotechnology Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Monomer chemistry 030220 oncology & carcinogenesis Microseminoprotein-beta Electrode Target protein 0210 nano-technology Biosensor |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2015.09.133 |
| Popis: | In the past few years a large effort is being made aiming at the development of fast and reliable tests for cancer biomarkers. Protein imprinted sensors can be a fast and reliable strategy to develop tailor made sensors for a large number of relevant molecules. This work aims to produce, optimize and use in biological samples a biosensor for microseminoprotein-beta (MSMB). Caffeic acid (CAF) electropolimerization was performed in the presence of microseminoprotein-beta (MSMB) creating target protein specific cavities on the surface of a screen-printed carbon. Dopamine was introduced as charged monomer labelling the binding site and was allowed to self-organize around the protein. The subsequent electropolimerization was made by applying a constant potential of +2.0 V, for 30 s, on a carbon screen-printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer. The sensor with charged monomers showed a more sensitive response, with an average slope of −7.59 μA/decade, linear concentration range of 0.5–100 ng/mL and a detection limit of 0.12 ng/mL. The corresponding non-imprinted sensor displayed an inconsistent response over the range of the calibration curve. The biosensor was successfully applied to the analysis of MSMB in serum and urine samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect