Topical application of temoporfin-loaded invasomes for photodynamic therapy of subcutaneously implanted tumours in mice: A pilot study
| Autor: | Volker Albrecht, Alfred Fahr, Nina Dragicevic-Curic, Susanna Gräfe |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Drug
Skin Neoplasms Palliative treatment Administration Topical medicine.medical_treatment media_common.quotation_subject Biophysics Mice Nude Pilot Projects Photodynamic therapy Pharmacology Citral Temoporfin Mice chemistry.chemical_compound Oral carcinomas Cell Line Tumor Animals Humans Medicine Radiology Nuclear Medicine and imaging Photosensitizer media_common Liposome Photosensitizing Agents Radiation Ethanol Radiological and Ultrasound Technology Terpenes business.industry Tumor Burden Mesoporphyrins Photochemotherapy chemistry Liposomes Female business Neoplasm Transplantation |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 91:41-50 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/j.jphotobiol.2008.01.009 |
| Popis: | Temoporfin (mTHPC) represents a very potent second-generation synthetic photosensitizer. It has shown to be effective in the photodynamic therapy of early or recurrent oral carcinomas, in the palliative treatment of refractory oral carcinomas and in the treatment of primary non-melanomatous tumours of the skin of the head and neck. Until now for all positive findings an intravenous application of the photosensitizer was mandatory. In the case of cutaneous malignant or non-malignant diseases a topical application of the drug onto the site of the disease followed by illumination, would be advantageous. Unfortunately, mTHPC is a highly hydrophobic drug with a low percutaneous absorption. The purpose of this experiment was to investigate the photodynamic efficacy of novel mTHPC-loaded invasomes after their topical application onto the skin of mice bearing the subcutaneously implanted human colorectal tumour HT29 followed by photoirradiation. Invasomes are vesicles containing in addition to phospholipids a mixture of terpenes (cineole, citral and d-limonene) or only one terpene (citral) and ethanol, as penetration enhancers. This was a pilot study since until now no data are available about the efficacy of mTHPC in the photodynamic therapy of HT29 tumours after its topical application. The aim of this experiment was to investigate whether a mTHPC-loaded invasome formulation can reduce tumour size by photodynamic therapy or at least to find a formulation slowing down tumour growth compared to the control group (mice without any treatment). The groups of mice treated with mTHPC-invasomes containing 1% of the terpene mixture prior to photoirradiation showed a significantly smaller (p |
| Databáze: | OpenAIRE |
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