Selenium-deficient diet induces inflammatory response in the pig adrenal glands by activating TLR4/NF-κB pathway via miR-30d-R_1
| Autor: | Gu Xuedie, Zhang Kaixin, Liu Zhaoyi, Zhang Yiming, Pervez Ahmed Khoso, Li Shu, Lan Jing |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Swine Biophysics chemistry.chemical_element Inflammation medicine.disease_cause Biochemistry Antioxidants Biomaterials 03 medical and health sciences Selenium 0302 clinical medicine Selenium deficiency Internal medicine Adrenal Glands medicine Animals Receptor Metals and Alloys NF-kappa B medicine.disease Diet Blot Toll-Like Receptor 4 MicroRNAs Oxidative Stress 030104 developmental biology Endocrinology chemistry Chemistry (miscellaneous) 030220 oncology & carcinogenesis Tlr4 nf κb TLR4 medicine.symptom Oxidative stress |
| Zdroj: | Metallomics : integrated biometal science. 13(7) |
| ISSN: | 1756-591X |
| Popis: | Selenium (Se) is an important trace element to maintain the body's dynamic balance. Lack of Se can cause inflammation. Studies have shown that inflammation often leads to disorders of the hypothalamic–pituitary–adrenal axis, but the mechanism by which Se deficiency causes inflammation of the porcine adrenal glands is still unclear. In order to study the effect of Se deficiency on the adrenal glands of pigs, we obtained Se-deficient pig adrenal glands through a low-Se diet. The results of mass spectrometry showed that the Se content in the Se-deficient group was only one-tenth of the control group. We detected the expression of the toll-like receptor 4 (TLR4) and downstream factors by qRT-PCR and Western blotting, and found that the lack of Se affected the TLR4/NF-κB pathway. It is known that miR-155-3p, miR-30d-R_1, and miR-146b have all been verified for targeting relationship with TLR4. We confirmed by qRT-PCR that miR-30d-R_1 decreased most significantly in the Se-deficient pig model. Then we tested 25 selenoproteins and some indicators of oxidative stress. It is confirmed that Se deficiency reduces the antioxidant capacity and induces oxidative stress in pig adrenal tissue. In short, a diet lacking Se induces oxidative stress in pig adrenal tissues and leads to inflammation through the miR-30d-R_1/TLR4 pathway. This study provides a reference for the prevention of adrenal inflammation in pigs from a nutritional point of view. |
| Databáze: | OpenAIRE |
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