Whole-Genome sequencing of Trypanosoma brucei reveals introgression between subspecies that is associated with virulence
| Autor: | Mark J. Taylor, Paul Capewell, Annette MacLeod, Ian Goodhead, Michael L. Chance, Tanja Beament, Neil Hall, Suzanne Kay, Sarah Forrester, J. Wendi Bailey, Harry Noyes |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Trypanosoma brucei rhodesiense
Virulence Factors Molecular Sequence Data 030231 tropical medicine qu_475 Sequence Homology Virulence Trypanosoma brucei Subspecies Microbiology Disease Outbreaks 03 medical and health sciences 0302 clinical medicine Virology qx_70 parasitic diseases medicine Humans Uganda African trypanosomiasis Phylogeny 030304 developmental biology Recombination Genetic Genetics Whole genome sequencing 0303 health sciences biology Haplotype other Sequence Analysis DNA DNA Protozoan biology.organism_classification medicine.disease QR1-502 3. Good health Trypanosomiasis African qu_470 Genome Protozoan Trypanosomiasis Research Article |
| Zdroj: | mBio, Vol 4, Iss 4 (2013) mBio |
| ISSN: | 2150-7511 2161-2129 |
| Popis: | Human African trypanosomiasis is caused by two subspecies of Trypanosoma brucei. Trypanosoma brucei rhodesiense is found in East Africa and frequently causes acute disease, while Trypanosoma brucei gambiense is found in West Africa and is associated with chronic disease. Samples taken from a single focus of a Ugandan outbreak of T. b. rhodesiense in the 1980s were associated with either chronic or acute disease. We sequenced the whole genomes of two of these isolates, which showed that they are genetically distinct from each other. Analysis of single nucleotide polymorphism markers in a panel of 31 Ugandan isolates plus 32 controls revealed a mixture of East African and West African haplotypes, and some of these haplotypes were associated with the different virulence phenotypes. It has been shown recently that T. b. brucei and T. b. rhodesiense populations undergo genetic exchange in natural populations. Our analysis showed that these strains from the Ugandan epidemic were intermediate between the reference genome sequences of T. b. gambiense and T. b. brucei and contained haplotypes that were present in both subspecies. This suggests that the human-infective subspecies of T. brucei are not genetically isolated, and our data are consistent with genomic introgression between East African and West African T. b. brucei subspecies. This has implications for the control of the parasite, the spread of drug resistance, and understanding the variation in virulence and the emergence of human infectivity. IMPORTANCE We present a genetic study of the acute form of “sleeping sickness” caused by the protozoan parasite Trypanosoma brucei rhodesiense from a single outbreak in Uganda. This represents an advance in our understanding of the relationship between the T. b. rhodesiense and Trypanosoma brucei gambiense subspecies that have previously been considered geographically distinct. Our data suggest that introgression of West African-derived T. brucei haplotypes may be associated with differences in disease presentation in the East African disease. These findings are not only of scientific interest but also important for parasite control, as they suggest that the human-infective T. brucei subspecies are not genetically isolated. |
| Databáze: | OpenAIRE |
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