The ruminal bacterial community in lactating dairy cows has limited variation on a day-to-day basis
| Autor: | Joseph H. Skarlupka, Maria E. Kamenetsky, Kelsea A. Jewell, Garret Suen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
animal structures Short Report Biology Biochemistry 03 medical and health sciences Rumen Animal science Lactation medicine lcsh:SF1-1100 lcsh:Veterinary medicine 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science 030104 developmental biology medicine.anatomical_structure Rumen microbiota Bacterial 16S rRNA Next-generation sequencing lcsh:SF600-1100 Animal Science and Zoology Bacterial community lcsh:Animal culture Day to day Food Science Biotechnology |
| Zdroj: | Journal of Animal Science and Biotechnology, Vol 10, Iss 1, Pp 1-5 (2019) Journal of Animal Science and Biotechnology |
| ISSN: | 2049-1891 |
| Popis: | Dairy cows rely on a complex ruminal microbiota to digest their host-indigestible feed. Our ability to characterize this microbiota has advanced significantly due to developments in next-generation sequencing. However, efforts to sample the rumen, which typically involves removing digesta directly from the rumen via a cannula, intubation, or rumenocentesis, is costly and labor intensive. As a result, the majority of studies characterizing the rumen microbiota are conducted on samples collected at a single time point. Currently, it is unknown whether there is significant day-to-day variation in the rumen microbiota, a factor that could strongly influence conclusion drawn from studies that sample at a single time point. To address this, we examined day-to-day changes in the ruminal microbiota of lactating dairy cows using next-generation sequencing to determine if single-day sampling is representative of sampling across 3 consecutive days. We sequenced single-day solid and liquid fractions of ruminal digesta collected over 3 consecutive days from 12 cannulated dairy cows during the early, middle, and late stages of a single lactation cycle using the V4 region of the bacterial 16S rRNA gene. We then generated 97% similarity operational taxonomic units (OTUs) from these sequences and showed that any of the individual samples from a given 3-day sampling period is equivalent to the mean OTUs determined from the combined 3-d data set. This finding was consistent for both solid and liquid fractions of the rumen, and we thus conclude that there is limited day-to-day variability in the rumen microbiota. Electronic supplementary material The online version of this article (10.1186/s40104-019-0375-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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