Fibroblast-myocyte interactions in in vitro cardiomyogenesis
| Autor: | W.O. Gross |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Cell type
Myocardium Cell Cell Differentiation Heart Cell Biology Anatomy Cell Communication Chick Embryo Biology Fibroblasts Chick embryos In vitro Cell biology Culture Media medicine.anatomical_structure Intercellular Junctions Cell culture Cell Movement medicine Myocyte Animals Fibroblast Intracellular Cells Cultured |
| Zdroj: | Experimental cell research. 142(2) |
| ISSN: | 0014-4827 |
| Popis: | When in dissociated cell culture, heart muscle cells and fibroblasts taken from 8-day chick embryos do not remain unaffected by one another. Instead they interact from early incubation on. The interaction of dissociated heart cells appears to form the basis of cardiomyogenesis in vitro as obtained by various laboratories. Our cinematographs of monolayer cultures show six different phenomena: 1. When a fibroblast, wandering in a heart cell monolayer culture, enters the sphere of influence of a myocyte, contact between the two cell types occurs. To this effect the fibroblast changes its direction or its leading lamella ramifies. 2. The area of the muscle cell contacted by the fibroblast tends to dart out. The resulting myocyte process advances along the fibroblast. 3. Similar processes can be pulled out by the retracting fibroblast, and be guided passively to neighboring myocytes. 4. Thin threads produced in like manner and often repeatedly contacted by the handling lamella of the fibroblast subsequently broaden and develop into intercellular bridges. 5. A conspicuous reaction of myocytes after contact with fibroblasts is the accelerated initiation of the spreading phenomenon in which the muscle cell polarizes and starts to beat. 6. After contact, a myocyte can be transported to a neighboring muscle cell by a fibroblast. These six interactions result in the development of synchronously pulsating muscle cell units. |
| Databáze: | OpenAIRE |
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