An siRNA pathway prevents transgenerational retrotransposition in plants subjected to stress
| Autor: | Marie Mirouze, Hidetaka Ito, Hervé Gaubert, Etienne Bucher, Jerzy Paszkowski, Isabelle Vaillant |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Small interfering RNA STRESS Transcription Genetic Mutant Arabidopsis Retrotransposon 01 natural sciences Gene Expression Regulation Plant Gene Regulatory Networks RNA Small Interfering TEMPERATURE MUTATION Genetics Regulation of gene expression DNA Plant/genetics RNA Small Interfering/genetics METHODE D'ANALYSE 0303 health sciences Multidisciplinary Seedling/genetics ARN Arabidopsis Proteins/genetics ddc:580 RNA Plant REGULATION Retroelements/genetics DNA Plant Retroelements Heat-Shock Response/genetics PLANTE Biology Genes Plant Chromosomes Plant Genes Plant/genetics 03 medical and health sciences Extrachromosomal DNA Gene Expression Regulation Plant/genetics Epigenetics RETROTRANSPOSON Gene 030304 developmental biology Transcription Genetic/genetics Models Genetic Arabidopsis Proteins BIOLOGIE MOLECULAIRE biology.organism_classification GENE Arabidopsis/genetics/growth & development/physiology Mutagenesis Insertional Seedlings Mutagenesis Insertional/genetics Heat-Shock Response 010606 plant biology & botany Gene Regulatory Networks/genetics RNA Plant/genetics |
| Zdroj: | Nature, Vol. 472, No 7341 (2011) pp. 115-119 Nature |
| ISSN: | 0028-0836 |
| Popis: | Eukaryotic genomes consist to a significant extent of retrotransposons that are suppressed by host epigenetic mechanisms, preventing their uncontrolled propagation. However, it is not clear how this is achieved. Here we show that in Arabidopsis seedlings subjected to heat stress, a copia-type retrotransposon named ONSEN (Japanese 'hot spring') not only became transcriptionally active but also synthesized extrachromosomal DNA copies. Heat-induced ONSEN accumulation was stimulated in mutants impaired in the biogenesis of small interfering RNAs (siRNAs); however, there was no evidence of transposition occurring in vegetative tissues. After stress, both ONSEN transcripts and extrachromosomal DNA gradually decayed and were no longer detected after 20-30 days. Surprisingly, a high frequency of new ONSEN insertions was observed in the progeny of stressed plants deficient in siRNAs. Insertion patterns revealed that this transgenerational retrotransposition occurred during flower development and before gametogenesis. Therefore in plants with compromised siRNA biogenesis, memory of stress was maintained throughout development, priming ONSEN to transpose during differentiation of generative organs. Retrotransposition was not observed in the progeny of wild-type plants subjected to stress or in non-stressed mutant controls, pointing to a crucial role of the siRNA pathway in restricting retrotransposition triggered by environmental stress. Finally, we found that natural and experimentally induced variants in ONSEN insertions confer heat responsiveness to nearby genes, and therefore mobility bursts may generate novel, stress-responsive regulatory gene networks. |
| Databáze: | OpenAIRE |
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