Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus

Autor: Gert-Jan Godeke, P.A. van Rijn, J.M.A. Pol, Johanna Jacoba Maria Meulenberg, E. H. J. Wissink, H. A. R. van Wijk, Peter J. M. Rottier
Rok vydání: 2003
Předmět:
ID - Infectieziekten
Swine
medicine.drug_class
Monoclonal Antibody
receptor
monoclonal-antibodies
Porcine Reproductive and Respiratory Syndrome
Biology
Immunofluorescence
Monoclonal antibody
Cellular Protein
arteriviridae
Arterivirus
Mice
CIDC - Division Virology
Antigen
Virology
Macrophages
Alveolar
medicine
Animals
surface
Porcine respiratory and reproductive syndrome virus
lelystad virus
Glycoproteins
chemistry.chemical_classification
Mice
Inbred BALB C
Hybridomas
medicine.diagnostic_test
prrsv
Brief Report
CIDC - Divisie Virologie
Antibodies
Monoclonal
General Medicine
Radioimmunoprecipitation Assay
biology.organism_classification
medicine.anatomical_structure
chemistry
Cell Surface
Alveolar macrophage
Receptors
Virus
cells
Polypeptide
Pulmonary alveolus
protein
Glycoprotein
Alveolar Macrophage
Zdroj: Archives of Virology, 148(1), 177-187
Archives of Virology 148 (2003) 1
Archives of Virology
ISSN: 1432-8798
0304-8608
Popis: Summary. The aim of this study was to identify the receptor(s) for PRRSV on porcine alveolar macrophages (PAMs) by producing monoclonal antibodies (MAbs) against these cells. Hybridoma supernatants were selected for their ability to block PRRSV infection. Four MAbs, 1-8D2, 9.4C7, 9.9F2, and 3-3H2 inhibited infection and recognised cell surface, PAM-specific antigens as shown by immunofluorescence and immunoperoxidase monolayer assay. These MAbs were then used to identify cellular proteins involved in PRRSV infection by radioimmunoprecipitation assays (RIPAs). MAbs 1-8D2 and 9.9F2 each recognised a 150 kDa-polypeptide doublet, while MAbs 9.4C7 and 3-3H2 both recognised a 220 kDa-polypeptide. Glycosidase treatment demonstrated all these polypeptides to be N-glycosylated. Thus, multiple glycoproteins appear to be involved in infection of PAMs by PRRSV.
Databáze: OpenAIRE
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