Optimization of Enzymatic Synthesis of Betulinic Acid Amide in Organic Solvent by Response Surface Methodology (RSM)
| Autor: | Nor Suhaila Mohamad Hanapi, Rossuriati Dol Hamid, Siti Efliza Ashari, Nurul Atikah Binti Amin Yusof, Nursyamsyila Mat Hadzir |
|---|---|
| Přispěvatelé: | Research Management Institute of Universiti Teknologi MARA [600-IRMI/DANA5/3/LESTARI(0090/2016] |
| Rok vydání: | 2019 |
| Předmět: |
biology
Butylamine betulinic acid amide Substrate (chemistry) General Chemistry Novozym 435 central composite rotatable design (CCRD) response surface methodology (RSM) Catalysis Chemistry chemistry.chemical_compound chemistry Betulinic acid Yield (chemistry) Amide biology.protein Response surface methodology Lipase QD1-999 Nuclear chemistry |
| Zdroj: | Indonesian Journal of Chemistry, Vol 19, Iss 4, Pp 849-857 (2019) Indonesian Journal of Chemistry; Vol 19, No 4 (2019); 849-857 |
| ISSN: | 2460-1578 1411-9420 |
| DOI: | 10.22146/ijc.34903 |
| Popis: | Optimization of the lipase catalyzed enzymatic synthesis of betulinic acid amide in the presence of immobilized lipase, Novozym 435 from Candida antartica as a biocatalyst was studied. Response surface methodology (RSM) and 5-level-4-factor central-composite rotatable design (CCRD) were employed to evaluate the effects of the synthesis parameters, such as reaction time (20–36 h), reaction temperature (37–45 °C), substrate molar ratio of betulinic acid to butylamine (1:1–1:3), and enzyme amounts (80–120 mg) on the percentage yield of betulinic acid amide by direct amidation reaction. The optimum conditions for synthesis were: reaction time of 28 h 33 min, reaction temperature of 42.92 °C, substrate molar ratio of 1:2.21, and enzyme amount of 97.77 mg. The percentage yield of actual experimental values obtained 65.09% which compared well with the maximum predicted value of 67.23%. The obtained amide was characterized by GC, GCMS and 13C NMR. Betulinic acid amide (BAA) showed a better cytotoxicity compared to betulinic acid as the concentration inhibited 50% of the cell growth (IC50) against MDA-MB-231 cell line (IC50 < 30 µg/mL). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|