The permeation of dynorphin A 1–6 across the blood brain barrier and its effect on bovine brain microvessel endothelial cell monolayer permeability
Autor: | Susan M. Lunte, Kenneth L. Audus, Jane V. Aldrich, Courtney D. Kuhnline Sloan |
---|---|
Rok vydání: | 2012 |
Předmět: |
endocrine system
medicine.medical_specialty Cell Membrane Permeability Physiology Metabolite Neuropeptide Dynorphin Blood–brain barrier Dynorphins Biochemistry κ-opioid receptor Article Structure-Activity Relationship Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Microvessel Cells Cultured Brain Endothelial Cells Dynorphin A respiratory system Endothelial stem cell medicine.anatomical_structure chemistry Blood-Brain Barrier embryonic structures Cattle circulatory and respiratory physiology |
Zdroj: | Peptides. 38:414-417 |
ISSN: | 0196-9781 |
Popis: | Dynorphin A 1–17 (Dyn A 1–17) is an endogenous neuropeptide known to act at the kappa opioid receptor; it has been implicated in a number of neurological disorders, including neuropathic pain, stress, depression, and Alzheimer’s and Parkinson’s diseases. The investigation of Dyn A 1–17 metabolism at the blood-brain barrier (BBB) is important since the metabolites exhibit unique biological functions compared to the parent compound. In this work, Dyn A 1–6 is identified as a metabolite of Dyn A 1–17 in the presence of bovine brain microvessel endhothelial cells (BBMECs), using LC-MS/MS. The transport of Dyn A 1–6 at the BBB was examined using this in vitro cell culture model of the BBB. Furthermore, the permeation of the BBB by the low molecular weight, permeability marker fluorescein was characterized in the presence and absences of Dyn A 1–6. |
Databáze: | OpenAIRE |
Externí odkaz: |