Vitamin A deficiency modulates iron metabolism via ineffective erythropoiesis
| Autor: | Marcela de Sá Barreto da Cunha, Egle Machado de Almeida Siqueira, Sandra Fernandes Arruda, Luciano S. Trindade |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Erythrocytes Endocrinology Diabetes and Metabolism Interleukin-1beta Clinical Biochemistry Suppressor of Cytokine Signaling Proteins Kidney Biochemistry chemistry.chemical_compound Homeostasis Erythropoiesis Vitamin A Cation Transport Proteins chemistry.chemical_classification Nutrition and Dietetics medicine.diagnostic_test Vitamin A Deficiency Transferrin Iron Deficiencies Up-Regulation Liver Serum iron HAMP Genetic Markers Vitamin medicine.medical_specialty Iron Down-Regulation Biology Hepcidins Hepcidin Internal medicine medicine Animals RNA Messenger Rats Wistar Erythropoietin Molecular Biology Interleukin-6 Transferrin saturation medicine.disease Rats Vitamin A deficiency Endocrinology chemistry Suppressor of Cytokine Signaling 3 Protein Heme Oxygenase (Decyclizing) Immunology biology.protein Biomarkers Spleen |
| Zdroj: | The Journal of Nutritional Biochemistry. 25:1035-1044 |
| ISSN: | 0955-2863 |
| Popis: | Vitamin A modulates inflammatory status, iron metabolism and erythropoiesis. Given that these factors modulate the expression of the hormone hepcidin (Hamp), we investigated the effect of vitamin A deficiency on molecular biomarkers of iron metabolism, the inflammatory response and the erythropoietic system. Five groups of male Wistar rats were treated: control (AIN-93G), the vitamin A-deficient (VAD) diet, the iron-deficient (FeD) diet, the vitamin A- and iron-deficient (VAFeD) diet or the diet with 12 mg atRA/kg diet replacing all-trans-retinyl palmitate by all-trans retinoic acid (atRA). Vitamin A deficiency reduced serum iron and transferrin saturation levels, increased spleen iron concentrations, reduced hepatic Hamp and kidney erythropoietin messenger RNA (mRNA) levels and up-regulated hepatic and spleen heme oxygenase-1 gene expression while reducing the liver HO-1 specific activity compared with the control. The FeD and VAFeD rats exhibited lower levels of serum iron and transferrin saturation, lower iron concentrations in tissues and lower hepatic Hamp mRNA levels compared with the control. The treatment with atRA resulted in lower serum iron and transferrin concentrations, an increased iron concentration in the liver, a decreased iron concentration in the spleen and in the gut, and decreased hepatic Hamp mRNA levels. In summary, these findings suggest that vitamin A deficiency leads to ineffective erythropoiesis by the down-regulation of renal erythropoietin expression in the kidney, resulting in erythrocyte malformation and the consequent accumulation of the heme group in the spleen. Vitamin A deficiency indirectly modulates systemic iron homeostasis by enhancing erythrophagocytosis of undifferentiated erythrocytes. |
| Databáze: | OpenAIRE |
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