Vitamin A deficiency modulates iron metabolism via ineffective erythropoiesis

Autor: Marcela de Sá Barreto da Cunha, Egle Machado de Almeida Siqueira, Sandra Fernandes Arruda, Luciano S. Trindade
Rok vydání: 2014
Předmět:
Male
Erythrocytes
Endocrinology
Diabetes and Metabolism

Interleukin-1beta
Clinical Biochemistry
Suppressor of Cytokine Signaling Proteins
Kidney
Biochemistry
chemistry.chemical_compound
Homeostasis
Erythropoiesis
Vitamin A
Cation Transport Proteins
chemistry.chemical_classification
Nutrition and Dietetics
medicine.diagnostic_test
Vitamin A Deficiency
Transferrin
Iron Deficiencies
Up-Regulation
Liver
Serum iron
HAMP
Genetic Markers
Vitamin
medicine.medical_specialty
Iron
Down-Regulation
Biology
Hepcidins
Hepcidin
Internal medicine
medicine
Animals
RNA
Messenger

Rats
Wistar

Erythropoietin
Molecular Biology
Interleukin-6
Transferrin saturation
medicine.disease
Rats
Vitamin A deficiency
Endocrinology
chemistry
Suppressor of Cytokine Signaling 3 Protein
Heme Oxygenase (Decyclizing)
Immunology
biology.protein
Biomarkers
Spleen
Zdroj: The Journal of Nutritional Biochemistry. 25:1035-1044
ISSN: 0955-2863
Popis: Vitamin A modulates inflammatory status, iron metabolism and erythropoiesis. Given that these factors modulate the expression of the hormone hepcidin (Hamp), we investigated the effect of vitamin A deficiency on molecular biomarkers of iron metabolism, the inflammatory response and the erythropoietic system. Five groups of male Wistar rats were treated: control (AIN-93G), the vitamin A-deficient (VAD) diet, the iron-deficient (FeD) diet, the vitamin A- and iron-deficient (VAFeD) diet or the diet with 12 mg atRA/kg diet replacing all-trans-retinyl palmitate by all-trans retinoic acid (atRA). Vitamin A deficiency reduced serum iron and transferrin saturation levels, increased spleen iron concentrations, reduced hepatic Hamp and kidney erythropoietin messenger RNA (mRNA) levels and up-regulated hepatic and spleen heme oxygenase-1 gene expression while reducing the liver HO-1 specific activity compared with the control. The FeD and VAFeD rats exhibited lower levels of serum iron and transferrin saturation, lower iron concentrations in tissues and lower hepatic Hamp mRNA levels compared with the control. The treatment with atRA resulted in lower serum iron and transferrin concentrations, an increased iron concentration in the liver, a decreased iron concentration in the spleen and in the gut, and decreased hepatic Hamp mRNA levels. In summary, these findings suggest that vitamin A deficiency leads to ineffective erythropoiesis by the down-regulation of renal erythropoietin expression in the kidney, resulting in erythrocyte malformation and the consequent accumulation of the heme group in the spleen. Vitamin A deficiency indirectly modulates systemic iron homeostasis by enhancing erythrophagocytosis of undifferentiated erythrocytes.
Databáze: OpenAIRE