Anaphylaxis of small arteries: putative role of nitric oxide and prostanoids
| Autor: | Alė Laukevičienė, Paulius Uginčius, Egidijus Kėvelaitis, Robertas Lažauskas, Igor Korotkich |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Contraction (grammar) Endothelium Freund's Adjuvant Guinea Pigs anaphylaxis guinea pig endothelial relaxing factors nitric oxide indomethacin In Vitro Techniques Cyclooxygenase pathway Nitric oxide chemistry.chemical_compound Internal medicine medicine Animals Mesenteric arteries Analysis of Variance biology Chemistry Myography General Medicine Coronary Vessels Mesenteric Arteries Coronary arteries medicine.anatomical_structure Endocrinology Vasoconstriction Prostaglandins biology.protein cardiovascular system Endothelium Vascular Cyclooxygenase Hypotension Myograph |
| Zdroj: | Medicina; Volume 46; Issue 1; Pages: 38 Medicina Volume 46 Issue 1 |
| ISSN: | 1648-9144 |
| DOI: | 10.3390/medicina46010007 |
| Popis: | This study investigated possible implication of nitric oxide and prostanoids in anaphylactic reaction in small mesenteric and coronary arteries. Material and methods. Isolated arteries from guinea pigs, sensitized with 0.5 mL of horse serum or sham-sensitized, were challenged with 1% of horse serum in vitro. Contractile responses of arteries (normalized diameter, 350–450 μm) were recorded by a small blood vessel wire myograph. For inhibition of the release of NO or prostanoids, vessels were pretreated with N(G)-nitro-L-arginine methyl ester (30 μM) or indomethacin (10 μM), respectively. Results. Antigen challenge was followed by contraction of both coronary and mesenteric vessels. Two patterns of contraction were observed: 1) peak contraction – an immediate transient contraction of relatively high amplitude this was the most common pattern 2) biphasic: the initial peak contraction was followed by a slow growing contraction with low amplitude. Biphasic pattern was observed in 60% of the mesenteric vessels and 40% of the coronary vessels. Inhibition of NO synthase significantly increased the peak contraction in the coronary vessels and the second-phase contraction in the mesenteric vessels. Inhibition of cyclooxygenase caused a decrease in the peak and second-phase contraction of both the coronary and mesenteric vessels. Conclusions. Despite anaphylactic contraction, nitric oxide seems to be released from the endothelium following antigen challenge in the small coronary and mesenteric arteries. This may contribute to the development of hypotension during anaphylaxis. Prostanoids are playing a different role – the contracting products of cyclooxygenase pathway are important for the development of anaphylactic contraction of the small isolated arteries. |
| Databáze: | OpenAIRE |
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