Role of interferon gamma and tumour necrosis factor alpha in monocyte-mediated cytostasis and cytotoxicity against a human histiocytic lymphoma cell line
| Autor: | R.H.J. Beelen, Gert J. Ossenkoppele, Arjan A. van de Loosdrecht, Mart M.A.C. Langenhuijsen, Marjolein G. Broekhoven |
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| Rok vydání: | 1992 |
| Předmět: |
Cytotoxicity
Immunologic Cancer Research Time Factors Immunology Tetrazolium Salts Biology Immunotherapy Adoptive Monocytes Interferon-gamma medicine Tumor Cells Cultured Immunology and Allergy Cytotoxic T cell Humans Interferon gamma MTT assay Cytotoxicity Cell Death Tumor Necrosis Factor-alpha Monocyte Macrophages Cytostasis Recombinant Proteins Thiazoles medicine.anatomical_structure Oncology Monocyte differentiation Cancer research Tumor necrosis factor alpha Colorimetry Lymphoma Large B-Cell Diffuse medicine.drug |
| Zdroj: | Cancer immunology, immunotherapy : CII. 34(6) |
| ISSN: | 0340-7004 |
| Popis: | In view of cellular adoptive immunotherapy we have studied monocyte-mediated cytostasis and cytotoxicity against U 937 cells, a human histiocytic lymphoma cell line. Highly purified human monocytes and monocyte-derived macrophages were activated with interferon gamma (IFN) or tumour necrosis factor alpha (TNF) to antileukemic immune effector cells. Antileukemic activity of human monocytes was dependent on monocyte differentiation into macrophages and on a dose- and time-dependent activation with IFN or TNF. Maximum cytostasis of 97.0 +/- 0.7% (mean +/- SEM) (conventional [3H]dT uptake assay) and 81.9 +/- 5.3% cytotoxicity (modified MTT assay) of U 937 cells was obtained by monocytes activated with 100 U/ml IFN for at least 24 h at an effector-to-target-cell ratio of 10. U 937 cells premodified with IFN showed an increase in susceptibility to monocyte-mediated cytotoxicity. U 937 cells premodified with TNF were almost resistant to monocyte-mediated cytotoxicity while activated monocytes maintained their cytotoxic potential. These data show that IFN and TNF are potent activators of monocyte-mediated cytotoxicity. Furthermore, IFN and TNF might be involved in the regulation of the susceptibility of leukemic cells to lysis by interactions with monocytes or macrophages. |
| Databáze: | OpenAIRE |
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