Conservation of Imprinting and Methylation of MKRN3, MAGEL2 and NDN Genes in Cattle
| Autor: | Shukai Gu, Yanqiu Dong, Xiaoqian Liu, Dongjie Li, Shijie Li, Cui Zhang, Weina Chen, Junliang Li, Lanjie Jin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Veterinary medicine Bisulfite sequencing cow MKRN3 gene 030105 genetics & heredity Biology 03 medical and health sciences MAGEL2 gene SF600-1100 Imprinting (psychology) Gene NDN gene Genetics DNA methylation General Veterinary DMR Methylation 030104 developmental biology Differentially methylated regions QL1-991 Animal Science and Zoology imprinting Genomic imprinting Zoology SNRPN Gene |
| Zdroj: | Animals, Vol 11, Iss 1985, p 1985 (2021) Animals Volume 11 Issue 7 |
| ISSN: | 2076-2615 |
| Popis: | Genomic imprinting is the epigenetic mechanism of transcriptional regulation that involves differential DNA methylation modification. Comparative analysis of imprinted genes between species can help us to investigate the biological significance and regulatory mechanisms of genomic imprinting. MKRN3, MAGEL2 and NDN are three maternally imprinted genes identified in the human PWS/AS imprinted locus. This study aimed to assess the allelic expression of MKRN3, MAGEL2 and NDN and to examine the differentially methylated regions (DMRs) of bovine PWS/AS imprinted domains. An expressed single-nucleotide polymorphism (SNP)-based approach was used to investigate the allelic expression of MKRN3, MAGEL2 and NDN genes in bovine adult tissues and placenta. Consistent with the expression in humans and mice, we found that the MKRN3, MAGEL2 and NDN genes exhibit monoallelic expression in bovine somatic tissues and the paternal allele expressed in the bovine placenta. Three DMRs, PWS-IC, MKRN3 and NDN DMR, were identified in the bovine PWS/AS imprinted region by analysis of the DNA methylation status in bovine tissues using the bisulfite sequencing method and were located in the promoter and exon 1 of the SNRPN gene, NDN promoter and 5’ untranslated region (5’UTR) of MKRN3 gene, respectively. The PWS-IC DMR is a primary DMR inherited from the male or female gamete, but NDN and MKRN3 DMR are secondary DMRs that occurred after fertilization by examining the methylation status in gametes. |
| Databáze: | OpenAIRE |
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