Replication of MERS and SARS coronaviruses in bat cells offers insights to their ancestral origins
| Autor: | Ulrich Wernery, Kwok-Yung Yuen, Susanna K. P. Lau, Patrick C. Y. Woo, Kenneth S. M. Li, Joshua Fung, Syed Shakeel Ahmed, Longchao Zhu, Kin-Hang Kok, Jasper Fuk-Woo Chan, Rachel Y.Y. Fan, Kwok H. Chan, Emily Y.M. Wong, Hayes K.H. Luk |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Primates Human coronavirus 229E Camelus Middle East respiratory syndrome coronavirus Epidemiology Dipeptidyl Peptidase 4 viruses Immunology lcsh:QR1-502 Virus Attachment Biology medicine.disease_cause Virus Replication Microbiology lcsh:Microbiology Article lcsh:Infectious and parasitic diseases Cell Line 03 medical and health sciences Chiroptera Virology Drug Discovery medicine Animals Humans Pipistrellus lcsh:RC109-216 Rhinolophus sinicus Cells Cultured Phylogeny Genetics virus diseases General Medicine Tylonycteris respiratory system biology.organism_classification respiratory tract diseases Viral Tropism 030104 developmental biology Infectious Diseases Viral replication Severe acute respiratory syndrome-related coronavirus Cell culture Tylonycteris pachypus Spike Glycoprotein Coronavirus Tissue tropism Middle East Respiratory Syndrome Coronavirus Parasitology |
| Zdroj: | Emerging Microbes & Infections Emerging Microbes and Infections, Vol 7, Iss 1, Pp 1-11 (2018) |
| DOI: | 10.1101/326538 |
| Popis: | Previous findings of Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses in bats, and the ability ofTylonycteris-BatCoV HKU4 spike protein to utilize MERS-CoV receptor, human dipeptidyl peptidase 4 hDPP4, suggest a bat ancestral origin of MERS-CoV. We developed 12 primary bat cell lines from seven bat species, includingTylonycteris pachypus,Pipistrellus abramusandRhinolophus sinicus(hosts ofTylonycteris-BatCoV HKU4,Pipistrellus-BatCoV HKU5 and SARS-related-CoV respectively), and tested their susceptibilities to MERS-CoVs, SARS-CoV and human coronavirus 229E (HCoV-229E). Five cell lines, includingP. abramusandR. sinicusbut notT. pachypuscells, were susceptible to human MERS-CoV EMC/2012. However, three tested camel MERS-CoV strains showed different infectivities, with only two strains capable of infecting three and one cell lines respectively. SARS-CoV can only replicate inR. sinicuscells, while HCoV-229E cannot replicate in any bat cells. Bat dipeptidyl peptidase 4 (DPP4) sequences were closely related to those of human and non-human primates but distinct from dromedary DPP4 sequence. Critical residues for binding to MERS-CoV spike protein were mostly conserved in bat DPP4. DPP4 was expressed in the five bat cells susceptible to MERS-CoV, with significantly higher mRNA expression levels than those in non-susceptible cells (P=0.0174), supporting that DPP4 expression is critical for MERS-CoV infection in bats. However, overexpression ofT. pachypusDPP4 failed to confer MERS-CoV susceptibility inT. pachypuscells, suggesting other cellular factors in determining viral replication. The broad cellular tropism of MERS-CoV should prompt further exploration of host diversity of related viruses to identify its ancestral origin. |
| Databáze: | OpenAIRE |
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