Applicability of Preparative Overpressured Layer Chromatography and Direct Bioautography in Search of Antibacterial Chamomile Compounds
| Autor: | Péter G. Ott, Ágnes Alberti, Éva Lemberkovics, Ágnes Kéry, Andrea Böszörményi, Ágnes M. Móricz, Éva Szőke, Emil Mincsovics |
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| Rok vydání: | 2013 |
| Předmět: |
Pseudomonas syringae
Flowers Microbial Sensitivity Tests Fractionation Tandem mass spectrometry High-performance liquid chromatography Gas Chromatography-Mass Spectrometry Analytical Chemistry law.invention chemistry.chemical_compound Column chromatography Tandem Mass Spectrometry law Gram-Negative Bacteria Oils Volatile Environmental Chemistry Sample preparation Chromatography High Pressure Liquid Essential oil Pharmacology Chromatography Plant Extracts Chamomile Aliivibrio fischeri Anti-Bacterial Agents chemistry Adsorption Chromatography Thin Layer Methanol Gas chromatography–mass spectrometry Agronomy and Crop Science Chromatography Liquid Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 96:1214-1221 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.sgemoricz |
| Popis: | In situ sample preparation and preparative overpressured layer chromatography (OPLC) fractionation on a 0.5 mm thick adsorbent layer of chamomile flower methanol extract prepurified by conventional gravitation accelerated column chromatography were applied in searching for bioactive components. Sample cleanup in situ on the adsorbent layer subsequent to sample application was performed using mobile phase flow in the opposite direction (the input and output of the eluent was exchanged). The antibacterial effect of the fractions obtained from the stepwise gradient OPLC separation with the flow in the normal direction was evaluated by direct bioautography against two Gram-negative bacteria: the luminescence gene tagged plant pathogenic Pseudomonas syringae pv. maculicola, and the naturally luminescent marine bacterium Vibrio fischeri. The fractions having strong activity were analyzed by SPME-GC/MS and HPLC/MS/MS. Mainly essential oil components, coumarins, flavonoids, phenolic acids, and fatty acids were tentatively identified in the fractions. |
| Databáze: | OpenAIRE |
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