Autologous Peripheral Blood Mononuclear Cell Implantation for Patients With Peripheral Arterial Disease Improves Limb Ischemia
| Autor: | Mika Ueda, Shizuko Yakabi, Nobuhisa Yamane, Ayano Kakihana, Kageharu Koja, Kimihiro Komori, Yasushi Higashiuesato, Yusuke Ohya, Chiharu Miyagi, Katsuhiko Ohshiro, Akio Ishida, Shinobu Matsubara, Moriyasu Nakaema, Shuichi Takishita, Hitoshi Sakuda |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_treatment Ischemia Transplantation Autologous Peripheral blood mononuclear cell Angioplasty Granulocyte Colony-Stimulating Factor medicine Humans Peripheral Vascular Diseases Leg Arteriosclerosis obliterans business.industry Thromboangiitis Obliterans General Medicine Middle Aged medicine.disease Granulocyte colony-stimulating factor Peripheral Transplantation medicine.anatomical_structure Anesthesia Arm Leukocytes Mononuclear Bone marrow Cardiology and Cardiovascular Medicine business |
| Zdroj: | Circulation Journal. 69:1260-1265 |
| ISSN: | 1347-4820 1346-9843 |
| Popis: | Background Implantation of bone marrow mononuclear cells, including endothelial progenitor cells, into ischemic limbs has been shown to improve collateral vessel formation. In the present study the safety and feasibility of autologous peripheral blood mononuclear cells (PBMNCs) implantation after granulocyte-colony stimulating factor (G-CSF)-induced mobilization was investigated in patients with severe peripheral arterial disease. Methods and results Six cases were enrolled: 5 of thromboangitis obliterans and 1 of arteriosclerosis obliterans. Following administration of G-CSF (10 microg . kg(-1) . day(-1)), PBMNCs were harvested and injected intramuscularly (5 legs and 1 arm) for 2 days for the patients with ischemia of the legs. No serious adverse events related to G-CSF administration, harvest or implantation were observed during this study period. Improvement in the ankle - brachial pressure index (ABI: >0.1) was seen in 4 patients at 4 weeks and ischemic ulcers improved in 3 of 3 patients. The mean maximum walking distance significantly increased from 203 m to 559 m (p=0.031) at 4 weeks and was sustained for 24 weeks. Significant improvement was seen in physiological functioning subscale of Short Form-36. Conclusion Implantation of PBMNCs collected after G-CSF administration could be an alternative to therapeutic angioplasty in patients with severe peripheral arterial disease. |
| Databáze: | OpenAIRE |
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