DNA Relatedness between Field Isolates of Mycoplasma F38 Group, the Agent of Contagious Caprine Pleuropneumonia, and Strains of Mycoplasma capricolum
| Autor: | Joseph M. Bové, Ronald H. Leach, Colette Saillard, David L. Rose, Francoise Bonnet, Geoff S. Cottew, Joseph G. Tully |
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| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial 040301 veterinary sciences Immunology Fluorescent Antibody Technique Mycoplasmataceae Subspecies medicine.disease_cause Microbiology Mycoplasma capricolum 0403 veterinary science 03 medical and health sciences Nucleic acid thermodynamics Contagious caprine pleuropneumonia Mycoplasma Sequence Homology Nucleic Acid Genotype medicine Animals Pleuropneumonia Contagious 0303 health sciences Goat Diseases biology 030306 microbiology Goats Nucleic Acid Hybridization 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Mollicutes Genome Bacterial |
| Zdroj: | International Journal of Systematic Bacteriology. 43:597-602 |
| ISSN: | 1465-2102 0020-7713 |
| DOI: | 10.1099/00207713-43-3-597 |
| Popis: | DNA-DNA hybridization experiments were carried out in order to clarify the taxonomic relationships between the F38 group of caprine mycoplasmas, the established etiologic agents of classical contagious caprine pleuropneumonia, and Mycoplasma capricolum, an organism associated with septicemia, arthritis, and mastitis in goats and sheep. The taxonomic status of the F38 group has been uncertain, principally because of the serological, genomic, and other properties which it shares with M. capricolum. Tritium-labeled DNAs from the M. capricolum type strain (California kid) and from prototype strain F38 were hybridized with unlabeled DNAs from these two strains and from four other isolates belonging to each group. The results showed consistent DNA relatedness values of about 70% between the F38 and M. capricolum groups, compared with levels of relatedness of about 90 and 85%, respectively, for the strains within each group. In addition, the results of comparisons of these 10 strains in which growth inhibition and immunofluorescence tests were used confirmed the previously reported serological relationships between the two groups and reinforced other observations concerning their shared genomic and cell membrane characteristics, indicating that there is a close taxonomic relationship. However, as the 70% DNA relatedness values between the M. capricolum and F38 groups also indicate a degree of genomic difference inconsistent with a relationship at the species level, we conclude that our findings support previous proposals for classification of the F38 group as a subspecies of M. capricolum. In view of the prospective diagnostic problems, particularly those arising from the serological similarities of two putative subspecies, we believe that further studies should be performed to define additional phenotypic and genotypic properties that would allow more rapid and specific differentiation of the F38 group mycoplasmas. |
| Databáze: | OpenAIRE |
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