Studies on secondary metabolite profiling, anti-inflammatory potential, in vitro photoprotective and skin-aging related enzyme inhibitory activities of Malaxis acuminata , a threatened orchid of nutraceutical importance

Autor: Biswajit Bose, Hiranjit Choudhury, Pramod Tandon, Suman Kumaria
Rok vydání: 2017
Předmět:
0106 biological sciences
Xanthine Oxidase
Antioxidant
Ultraviolet Rays
DPPH
medicine.drug_class
medicine.medical_treatment
Anti-Inflammatory Agents
Biophysics
Secondary metabolite
01 natural sciences
Antioxidants
Gas Chromatography-Mass Spectrometry
Anti-inflammatory
chemistry.chemical_compound
Alkaloids
Nutraceutical
Botany
medicine
Radiology
Nuclear Medicine and imaging
Collagenases
Orchidaceae
Flavonoids
chemistry.chemical_classification
Radiation
Pancreatic Elastase
Radiological and Ultrasound Technology
biology
Traditional medicine
Monophenol Monooxygenase
Plant Extracts
010405 organic chemistry
Polyphenols
Glycoside
biology.organism_classification
Skin Aging
0104 chemical sciences
Plant Leaves
chemistry
Polyphenol
Dietary Supplements
Sunscreening Agents
010606 plant biology & botany
medicine.drug
Zdroj: Journal of Photochemistry and Photobiology B: Biology. 173:686-695
ISSN: 1011-1344
Popis: Malaxis acuminata D. Don., a small, terrestrial orchid, is endemic to tropical Himalayas at an altitude of 1200-2000m asl. The dried pseudobulbs are important ingredients of century old ayurvedic drug 'Ashtavarga' and a polyherbal immune-booster nutraceutical 'Chyavanprash', known to restore vigour, vitality and youthfulness. Considering tremendous medicinal importance of this threatened orchid species, a detailed study was undertaken for the first time to address its antioxidant potential, secondary metabolite contents and biological activities against skin-aging related enzymes (anti-collagenase, anti-elastase, anti-tyrosinase and xanthine oxidase) and anti-inflammatory activity (5-lipoxygenase and hyaluronidase) in different plant parts of wild and in vitro-derived plants of M. acuminata. Methanolic leaf and stem extracts were further evaluated for in vitro photoprotective activity against UV-B and UV-A radiations. Furthermore, secondary metabolite profiling of various plant parts was carried out by Gas Chromatography Mass Spectrometry (GC-MS). A significantly higher antioxidant potential (DPPH, metal chelating and ABTS•+) with a comparative higher yield of secondary metabolites was observed in in vitro-derived plantlets as compared to the wild plants. Among various solvent systems used, methanolic leaf and stem extracts showed promising inhibitory activity against major skin aging-related enzymes and anti-inflammatory potential. Methanolic leaf and stem extracts of both wild and in vitro-derived plants showed promising photoprotective activity against UV-B and UV-A radiations in vitro with comparatively higher sun protection factor (SPF). Furthermore, GC-MS analysis of methanolic extracts of leaves and stems of wild as well as in vitro-derived plantlets revealed presence of many bioactive metabolites such as, dietary fatty acids, α-hydroxy acids, phenolic acids, sterols, amino acids, sugars and glycosides which substantially explain the use of M. acuminata as one of the potential rejuvenator and anti-aging ingredient in many Ayurvedic formulations.
Databáze: OpenAIRE
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