Cortex-wide neural interfacing via transparent polymer skulls
| Autor: | Madelyn M Gray, Mathew L. Rynes, Kevin W. Eliceiri, Suhasa B. Kodandaramaiah, Nahom Mossazghi, Gang Chen, Sarah West, Anant Naik, Jia Hu, Leila Ghanbari, Judith Dominguez, Abdul Kader Sagar, Timothy J. Ebner, Lenora Haltom, Russell E. Carter |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Dorsum Male Computer science Polymers media_common.quotation_subject Science General Physics and Astronomy 02 engineering and technology General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Neural activity Mice Calcium imaging 0302 clinical medicine Neuroimaging Perception Cortex (anatomy) medicine Animals lcsh:Science 030304 developmental biology media_common Cerebral Cortex 0303 health sciences Multidisciplinary Skull Brain food and beverages General Chemistry 021001 nanoscience & nanotechnology Mice Inbred C57BL Electrophysiology 030104 developmental biology medicine.anatomical_structure Cerebral cortex Interfacing lcsh:Q Calcium 0210 nano-technology Neuroscience 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Neural computations occurring simultaneously in multiple cerebral cortical regions are critical for mediating behaviors. Progress has been made in understanding how neural activity in specific cortical regions contributes to behavior. However, there is a lack of tools that allow simultaneous monitoring and perturbing neural activity from multiple cortical regions. We engineered ‘See-Shells’—digitally designed, morphologically realistic, transparent polymer skulls that allow long-term (>300 days) optical access to 45 mm2 of the dorsal cerebral cortex in the mouse. We demonstrate the ability to perform mesoscopic imaging, as well as cellular and subcellular resolution two-photon imaging of neural structures up to 600 µm deep. See-Shells allow calcium imaging from multiple, non-contiguous regions across the cortex. Perforated See-Shells enable introducing penetrating neural probes to perturb or record neural activity simultaneously with whole cortex imaging. See-Shells are constructed using common desktop fabrication tools, providing a powerful tool for investigating brain structure and function. Imaging the mouse brain using glass cranial windows has limitations in terms of flexibility and long-term imaging. Here the authors engineer transparent polymer skulls that can fit various skull morphologies and can be implanted for over 300 days, enabling simultaneous high resolution brain imaging and electrophysiology across large cortical areas. |
| Databáze: | OpenAIRE |
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