RhoA and Rac mediate endothelial cell polarization and detachment induced by T-cadherin
| Autor: | Paul Erne, Yoh Takuwa, Maria Philippova, Thérèse J. Resink, Roy Allenspach, Danila Ivanov |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
rac1 GTP-Binding Protein
Umbilical Veins RHOA Myosin Light Chains Biology Protein Serine-Threonine Kinases Transfection Biochemistry Genetics Cell Adhesion Humans cardiovascular diseases Phosphorylation Cell adhesion Molecular Biology Cells Cultured Cytoskeleton Cell Size rho-Associated Kinases Cadherin Cell adhesion molecule Intracellular Signaling Peptides and Proteins Cell Polarity Endothelial Cells Cell migration Cadherins Recombinant Proteins Cell biology rac GTP-Binding Proteins Endothelial stem cell T-cadherin Enzyme Activation Microscopy Fluorescence Mutation biology.protein rhoA GTP-Binding Protein Intracellular Biotechnology Signal Transduction |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 19(6) |
| ISSN: | 1530-6860 |
| Popis: | T-cadherin (T-cad) is an atypical GPI-anchored member of the cadherin superfamily. Ligation of T-cad receptors on endothelial cells prevents cell spreading, promotes elongation and polarization, decreases adhesion to the matrix, and facilitates migration. This study investigates involvement of Rho GTPases in T-cad signaling. Human umbilical vein endothelial cells were infected with adenoviral vectors expressing dominant-negative and/or constitutively active mutants of RhoA (N19RhoA/RhoA63), ROCK (RB/PH(TT)/CAT), and Rac1 (N17RAC). Mutant-infected and empty vector-infected cells were compared with respect to their ability to detach and polarize when plated on substratum containing recombinant T-cad protein used as a ligand mimicking homophilic T-cad interactions. ROCK involvement was also studied using specific inhibitor Y-27632. Adhesion assays, analysis of cell phenotype, and actin cytoskeleton organization using TRITC-labeled phalloidin demonstrated that T-cad-induced cell polarization includes two complementary components: RhoA/ROCK pathway is necessary for cell contraction, stress fiber assembly, and inhibition of spreading, whereas Rac is required for formation of actin-rich lamellipodia at the leading edges of polarized cells. Individual repression of either pathway only partially prevented cell polarization and detachment, while simultaneous repression of RhoA and Rac pathways fully eliminated responses to homophilic T-cad ligation. In conclusion, these data suggest that T-cad induces cell deadhesion and polarization via RhoA-ROCK- and Rac-dependent mechanisms. |
| Databáze: | OpenAIRE |
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