An isotope dilution capillary electrophoresis/tandem mass spectrometry (CE-MS/MS) method for the simultaneous measurement of choline, betaine, and dimethylglycine concentrations in human plasma
| Autor: | Ciriaco Carru, Stefano Assaretti, Mauro Forteschi, Gianfranco Pintus, Salvatore Sotgia, Angelo Zinellu, Arduino A. Mangoni |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Electrospray ionization Indicator Dilution Techniques Isotope dilution Tandem mass spectrometry Mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry Choline Dimethylglycine 03 medical and health sciences chemistry.chemical_compound Betaine Capillary electrophoresis Limit of Detection Tandem Mass Spectrometry Humans Aged Chromatography 010401 analytical chemistry Selected reaction monitoring Electrophoresis Capillary Sarcosine Middle Aged 0104 chemical sciences 030104 developmental biology chemistry Isotope Labeling Female |
| Zdroj: | Analytical and bioanalytical chemistry. 408(26) |
| ISSN: | 1618-2650 |
| Popis: | Plasma concentrations of choline, betaine, and dimethylglycine provide valuable information on the flow of methyl groups in key biological processes, particularly during folate deficiency states. We developed a new method to simultaneously measure these analytes in human plasma. Following sample deproteinization using acetonitrile, an aliquot was evaporated to dryness under vacuum to be then taken up by water. Finally, analytes were separated by capillary electrophoresis and detected by electrospray ionization triple-quadrupole mass spectrometry, in multiple reaction monitoring mode, using two stable isotope-labeled internal standards. Linearity of the calibration curves of each analyte was good (R(2) > 0.99). Average limits of detection (LODs) and limits of quantification (LOQs) for choline, betaine, and dimethylglycine were, respectively, 0.43, 0.62, and 0.31 μmol/L and 1.52, 2.11, and 0.97 μmol/L. Mean recovery of three replicates of two spiked concentrations levels was close to 100 % for all of the analytes. Repeatability and intermediate precision, expressed as %RSD of measurements, were |
| Databáze: | OpenAIRE |
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