Identification and characterization of microsatellite markers for population genetic studies of Panstrongylus megistus (Burmeister, 1835) (Triatominae: Reduviidae)
| Autor: | Fabiano Sviatopolk-Mirsky Pais, Liléia Diotaiuti, Flávio Campos Ferreira, Jeronimo C. Ruiz, Leonardo Barbosa Koerich, Leilane Oliveira Gonçalves, Carlota Josefovicz Belisário |
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| Rok vydání: | 2021 |
| Předmět: |
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0301 basic medicine 030231 tropical medicine Population Short Report Infectious and parasitic diseases RC109-216 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Molecular marker Animals Chagas Disease Allele Microsatellites education Triatominae education.field_of_study Contig biology Sequence Analysis DNA Panstrongylus biology.organism_classification Panstrongylus megistus Insect Vectors genomic DNA Genetics Population 030104 developmental biology Infectious Diseases Reduviidae chemistry Research Design Evolutionary biology Microsatellite Female Parasitology Brazil Microsatellite Repeats |
| Zdroj: | Parasites & Vectors, Vol 14, Iss 1, Pp 1-8 (2021) Parasites & Vectors |
| ISSN: | 1756-3305 |
| Popis: | Background Panstrongylus megistus is the most important vector of Chagas disease in Brazil. Studies show that the principal factor hindering the control of triatomines is reinfestation of houses previously treated with insecticides. Studies at the microgeographic level are therefore necessary to better understand these events. However, an efficient molecular marker is not yet available for carrying out such analyses in this species. The aim of the present study was to identify and characterize microsatellite loci for future population genetic studies of P. megistus. Methods This study work consisted of five stages: (i) sequencing of genomic DNA; (ii) assembly and selection of contigs containing microsatellites; (iii) validation of amplification and evaluation of polymorphic loci; (iv) standardization of the polymorphic loci; and (v) verification of cross-amplification with other triatomine species. Results Sequencing of males and females generated 7,908,463 contigs with a total length of 2,043,422,613 bp. A total of 2,043,690 regions with microsatellites in 1,441,091 contigs were obtained, with mononucleotide repeats being the most abundant class. From a panel of 96 loci it was possible to visualize polymorphisms in 64.55% of the loci. Of the 20 loci genotyped, the number of alleles varied from two to nine with an average of 4.9. Cross-amplification with other species of triatomines was observed in 13 of the loci. Conclusions Due to the high number of alleles encountered, polymorphism and the capacity to amplify from geographically distant populations, the microsatellites described here show promise for utilization in population genetic studies of P. megistus. Graphical Abstract |
| Databáze: | OpenAIRE |
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