F-Type Bacteriocins of Listeria monocytogenes: a New Class of Phage Tail-Like Structures Reveals Broad Parallel Coevolution between Tailed Bacteriophages and High-Molecular-Weight Bacteriocins
| Autor: | Dana Gebhart, Urmi Chakraborty, Dean Scholl, Grace J. Lee, Gregory R. Govoni, Z. Hong Zhou |
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| Přispěvatelé: | Silhavy, TJ |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology Human pathogen Bacillus subtilis medicine.disease_cause Medical and Health Sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Listeria monocytogenes Bacteriocin Bacteriocins medicine Genetics Bacteriophages Molecular Biology biology Agricultural and Veterinary Sciences Pseudomonas aeruginosa Viral Tail Proteins Articles Biological Sciences biology.organism_classification Foodborne Illness Biological Evolution Molecular Weight chemistry bacteria Digestive Diseases DNA Function (biology) |
| Zdroj: | Journal of bacteriology, vol 198, iss 20 |
| Popis: | Listeria monocytogenes is a significant foodborne human pathogen that can cause severe disease in certain high-risk individuals. L. monocytogenes is known to produce high-molecular-weight, phage tail-like bacteriocins, or “monocins,” upon induction of the SOS system. In this work, we purified and characterized monocins and found them to be a new class of F-type bacteriocins. The L. monocytogenes monocin genetic locus was cloned and expressed in Bacillus subtilis , producing specifically targeted bactericidal particles. The receptor binding protein, which determines target cell specificity, was identified and engineered to change the bactericidal spectrum. Unlike the F-type pyocins of Pseudomonas aeruginosa , which are related to lambda-like phage tails, monocins are more closely related to TP901-1-like phage tails, structures not previously known to function as bacteriocins. Monocins therefore represent a new class of phage tail-like bacteriocins. It appears that multiple classes of phage tails and their related bacteriocins have coevolved separately in parallel. IMPORTANCE Phage tail-like bacteriocins (PTLBs) are structures widespread among the members of the bacterial kingdom that are evolutionarily related to the DNA delivery organelles of phages (tails). We identified and characterized “monocins” of Listeria monocytogenes and showed that they are related to the tail structures of TP901-1-like phages, structures not previously known to function as bacteriocins. Our results show that multiple types of envelope-penetrating machines have coevolved in parallel to function either for DNA delivery (phages) or as membrane-disrupting bacteriocins. While it has commonly been assumed that these structures were coopted from phages, we cannot rule out the opposite possibility, that ancient phages coopted complex bacteriocins from the cell, which then underwent adaptations to become efficient at translocating DNA. |
| Databáze: | OpenAIRE |
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