Replication of ICP0-Null Mutant Herpes Simplex Virus Type 1 Is Restricted by both PML and Sp100▿
| Autor: | Philippe Gripon, Roger D. Everett, Anne Orr, Carlos Parada, Hüseyin Sirma |
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| Přispěvatelé: | MRC Virology Unit, Institute of Virology, Signalisation et Réponses aux Agents Infectieux et Chimiques (SeRAIC), Université de Rennes (UR), Régulations des équilibres fonctionnels du foie normal et pathologique, Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Heinriche-Pette-Institute, the DFG, Stiftung für neurovirale Erkrankungen to H.S, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Neoplasm Proteins
viruses Mutant Fluorescent Antibody Technique Gene Expression Herpesvirus 1 Human Viral Plaque Assay MESH: Leukopenia MESH: Base Sequence Promyelocytic Leukemia Protein Virus Replication Autoantigens MESH: Aged 80 and over MESH: Autoimmune Diseases Gene expression MESH: Microscopy Confocal Nuclear protein MESH: Fluorescent Antibody Technique MESH: Aged 0303 health sciences MESH: Middle Aged MESH: Receptors Antigen T-Cell alpha-beta Microscopy Confocal biology 030302 biochemistry & molecular biology Nuclear Proteins Antigens Nuclear MESH: Transcription Factors MESH: Splenomegaly 3. Good health Ubiquitin ligase Virus-Cell Interactions Neoplasm Proteins MESH: Herpesvirus 1 Human MESH: Viral Plaque Assay MESH: Autoantigens [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Histone deacetylase activity MESH: Leukemia T-Cell medicine.drug MESH: DNA Primers MESH: Gene Expression MESH: Mutation MESH: Immunophenotyping Ubiquitin-Protein Ligases Immunology Microbiology Immediate early protein Cell Line Immediate-Early Proteins 03 medical and health sciences Promyelocytic leukemia protein MESH: Receptors Antigen T-Cell gamma-delta Virology medicine Humans MESH: Tumor Suppressor Proteins MESH: Antigens Nuclear 030304 developmental biology DNA Primers MESH: Humans Base Sequence Tumor Suppressor Proteins MESH: Virus Replication MESH: Clone Cells MESH: Adult MESH: Immediate-Early Proteins biochemical phenomena metabolism and nutrition MESH: Ubiquitin-Protein Ligases Molecular biology MESH: Male MESH: Cell Line MESH: Gene Rearrangement gamma-Chain T-Cell Antigen Receptor Trichostatin A Insect Science Mutation biology.protein MESH: Nuclear Proteins MESH: Female Transcription Factors |
| Zdroj: | Journal of Virology Journal of Virology, 2008, 82 (6), pp.2661-72. ⟨10.1128/JVI.02308-07⟩ Journal of Virology, American Society for Microbiology, 2008, 82 (6), pp.2661-72. ⟨10.1128/JVI.02308-07⟩ |
| ISSN: | 0022-538X 1098-5514 |
| DOI: | 10.1128/JVI.02308-07⟩ |
| Popis: | Herpes simplex virus type 1 (HSV-1) mutants that fail to express the viral immediate-early protein ICP0 have a pronounced defect in viral gene expression and plaque formation in limited-passage human fibroblasts. ICP0 is a RING finger E3 ubiquitin ligase that induces the degradation of several cellular proteins. PML, the organizer of cellular nuclear substructures known as PML nuclear bodies or ND10, is one of the most notable proteins that is targeted by ICP0. Depletion of PML from human fibroblasts increases ICP0-null mutant HSV-1 gene expression, but not to wild-type levels. In this study, we report that depletion of Sp100, another major ND10 protein, results in a similar increase in ICP0-null mutant gene expression and that simultaneous depletion of both proteins complements the mutant virus to a greater degree. Although chromatin assembly and modification undoubtedly play major roles in the regulation of HSV-1 infection, we found that inhibition of histone deacetylase activity with trichostatin A was unable to complement the defect of ICP0-null mutant HSV-1 in either normal or PML-depleted human fibroblasts. These data lend further weight to the hypothesis that ND10 play an important role in the regulation of HSV-1 gene expression. |
| Databáze: | OpenAIRE |
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