Improved Methods to Produce Tissue-Engineered Skin Substitutes Suitable for the Permanent Closure of Full-Thickness Skin Injuries
| Autor: | Akram Ayoub, Laurence Cantin-Warren, Danielle Larouche, Maxime Desgagné, François A. Auger, Robert Gauvin, Israël Martel, Amélie Lavoie, Véronique J. Moulin, Lucie Germain, Rina Guignard |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty transplants Materials science 0206 medical engineering regenerative medicine lcsh:Medicine 02 engineering and technology autologous Regenerative medicine skin equivalent General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Tissue culture Skin substitutes medicine Full thickness skin Skin equivalent Original Research Article tissue culture lcsh:QH301-705.5 lcsh:R tissue therapy 020601 biomedical engineering Surgery 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Tissue engineered skin Seeding Keratinocyte Biomedical engineering |
| Zdroj: | BioResearch Open Access, Vol 5, Iss 1, Pp 320-329 (2016) BioResearch Open Access |
| ISSN: | 2164-7860 2164-7844 |
| DOI: | 10.1089/biores.2016.0036 |
| Popis: | There is a clinical need for skin substitutes to replace full-thickness skin loss. Our group has developed a bilayered skin substitute produced from the patient's own fibroblasts and keratinocytes referred to as Self-Assembled Skin Substitute (SASS). After cell isolation and expansion, the current time required to produce SASS is 45 days. We aimed to optimize the manufacturing process to standardize the production of SASS and to reduce production time. The new approach consisted in seeding keratinocytes on a fibroblast-derived tissue sheet before its detachment from the culture plate. Four days following keratinocyte seeding, the resulting tissue was stacked on two fibroblast-derived tissue sheets and cultured at the air–liquid interface for 10 days. The resulting total production time was 31 days. An alternative method adapted to more contractile fibroblasts was also developed. It consisted in adding a peripheral frame before seeding fibroblasts in the culture plate. SASSs produced by both new methods shared similar histology, contractile behavior in vitro and in vivo evolution after grafting onto mice when compared with SASSs produced by the 45-day standard method. In conclusion, the new approach for the production of high-quality human skin substitutes should allow an earlier autologous grafting for the treatment of severely burned patients. |
| Databáze: | OpenAIRE |
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