Novel Microarray-Based Method for Estimating Exposure to Ionizing Radiation
| Autor: | Gaëtan Gruel, Alexandre Pawlik, Olivier Alibert, Beatrice Jacquelin, Xavier Gidrol, Carlo Lucchesi, Anissa Zarour, Diana Tronik-LeRoux, Thierry Kortulewski, Vincent Frouin |
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| Přispěvatelé: | Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), This work was supported by funds from the Commisariat à l'Energie Atomique (CEA) and Electricité de France (EDF), GG was also supported by a predoctoral grant of the Association pour la Recherche sur le Cancer (ARC), AP by a predoctoral grant of the Fondation pour la Recherche Médicale (FRM), and BJ by a postdoctoral training grant from Genopole©-Evry., We would like to thank C. Joubert, P Flamant and V. Neuville for outstanding work in animal care, G. Munhoz for helpful discussions, and P. Soularue, A. Robert and P. Maltere for producing the cDNA microarray slides. |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
Microarray Gene Expression Ionizing radiation Mice 0302 clinical medicine Radiation Ionizing MESH: Radiometry / methods MESH: Body Burden Gene expression MESH: Animals Oligonucleotide Array Sequence Analysis 0303 health sciences Radiation MESH: Relative Biological Effectiveness Blood Proteins 030220 oncology & carcinogenesis Body Burden Algorithms MESH: Radiation Dosage Biophysics Computational biology Biology MESH: Radiation Ionizing Radiation Dosage 03 medical and health sciences MESH: Mice Inbred C57BL [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] MESH: Dose-Response Relationship Radiation Animals MESH: Gene Expression / radiation effects Radiology Nuclear Medicine and imaging Epigenetics Radiometry Gene MESH: Mice 030304 developmental biology Dosimeter business.industry Dose-Response Relationship Radiation Environmental Exposure MESH: Male Mice Inbred C57BL Gene selection MESH: Environmental Exposure / analysis MESH: Algorithms Gene chip analysis MESH: Blood Proteins / analysis MESH: Oligonucleotide Array Sequence Analysis / methods Nuclear medicine business Relative Biological Effectiveness |
| Zdroj: | Radiation Research Radiation Research, 2006, 166 (5), pp.746-756. ⟨10.1667/rr0260.1⟩ Radiation Research, Radiation Research Society, 2006, 166 (5), pp.746-756. ⟨10.1667/rr0260.1⟩ |
| ISSN: | 0033-7587 |
| DOI: | 10.1667/rr0260.1⟩ |
| Popis: | International audience; Accurate estimation of the dose of ionizing radiation to which individuals have been exposed is critical for therapeutic treatment. We investigated whether gene expression profiles could be used to evaluate the dose received, thereby serving as a biological dosimeter. We used cDNA microarrays to monitor changes in gene expression profiles induced by ionizing radiation in mouse total blood. The subsets of genes best characterizing each dose were identified by resampling the original data set and calculating the intersection of the dose signatures. This analytical strategy minimizes the impact of potential genetic/epigenetic variation between mice and overcomes the bias in gene selection inherent to microarray technology. The significance of the identified signatures was evaluated by monitoring the type I error rate by in silico negative control simulation. Based on the distribution of the mean ratios of the selected probes, we were able to identify transcription profiles giving 83% to 100% correct estimation of the dose received by test mice, demonstrating that the selected probes could be used to determine the dose of radiation to which the animals had been exposed. This method could potentially be generalized to determine the level of exposure to other toxins and could be used to develop new related clinical applications. |
| Databáze: | OpenAIRE |
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