Targeting of vascular adhesion protein-1 by positron emission tomography visualizes sites of inflammation in Borrelia burgdorferi-infected mice

Autor: Annukka Pietikäinen, Riikka Siitonen, Meeri Käkelä, Jukka Hytönen, Anne Roivainen, Mirva Söderström, Heidi Liljenbäck, Sirpa Jalkanen
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Pathology
medicine.medical_specialty
lcsh:Diseases of the musculoskeletal system
030106 microbiology
Arthritis
VAP-1
Inflammation
Gallium Radioisotopes
03 medical and health sciences
Mice
Lyme disease
Internal medicine
Positron Emission Tomography Computed Tomography
medicine
Animals
Humans
Borrelia burgdorferi
Lyme borreliosis
Sialic Acid Binding Immunoglobulin-like Lectins
Lyme Disease
biology
medicine.diagnostic_test
business.industry
Ceftriaxone
Siglec-9
medicine.disease
biology.organism_classification
Immunohistochemistry
Tibiotarsal joint
Rheumatology
3. Good health
Anti-Bacterial Agents
Disease Models
Animal
030104 developmental biology
PET
arthritis
Positron emission tomography
Host-Pathogen Interactions
Amine Oxidase (Copper-Containing)
medicine.symptom
lcsh:RC925-935
business
Infection
Cell Adhesion Molecules
Research Article
Zdroj: Arthritis Research & Therapy, Vol 19, Iss 1, Pp 1-10 (2017)
Arthritis Research & Therapy
ISSN: 1478-6362
DOI: 10.1186/s13075-017-1460-4
Popis: Background In the present study, we sought to evaluate the feasibility of targeting vascular adhesion protein-1 (VAP-1) by positron emission tomography (PET) for the longitudinal quantitative assessment of Borrelia burgdorferi infection-induced inflammation in mice. Methods Mice with B. burgdorferi infection-induced arthritis were studied. During a 7-week follow-up period, the progression of arthritis was monitored weekly with 68Ga-DOTA-Siglec-9 PET/computed tomography (CT) and measurement of tibiotarsal joint swellings. A subgroup of infected mice was treated with ceftriaxone. Finally, histopathological assessment of joint inflammation was performed and VAP-1 expression in joints were determined. Results Explicit joint swelling and 68Ga-DOTA-Siglec-9 uptake could be demonstrated in the affected joints from B. burgdorferi-infected mice. By contrast, no obvious accumulation of 68Ga-DOTA-Siglec-9 was detected in joints of uninfected mice. The maximum swelling and highest uptake in the affected joints were observed 4 weeks after the infection. 68Ga-DOTA-Siglec-9 uptake in joints correlated with joint swelling (P
Databáze: OpenAIRE
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