Anti-Epithelial Cell Adhesion Molecule Antibodies and the Detection of Circulating Normal-Like Breast Tumor Cells
| Autor: | Anieta M. Sieuwerts, Jaco Kraan, Joan Bolt-De Vries, Petra Van Der Spoel, Fons Elstrodt, Marcel Smid, Mieke Timmermans, Bianca Mostert, Mieke Schutte, John W. M. Martens, Jan-Willem Gratama, Stefan Sleijfer, John A. Foekens |
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| Přispěvatelé: | Medical Oncology, Pathology |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
CA15-3
Pathology medicine.medical_specialty Cancer Research Antibodies Neoplasm Breast Neoplasms Biology Brief Communication Metastasis chemistry.chemical_compound Circulating tumor cell SDG 3 - Good Health and Well-being Cancer stem cell Antigens Neoplasm Predictive Value of Tests Cell Line Tumor medicine Biomarkers Tumor Humans Gene Expression Profiling Cancer Epithelial cell adhesion molecule medicine.disease Epithelial Cell Adhesion Molecule Neoplastic Cells Circulating chemistry Oncology Research Design Cancer cell Female Breast disease Cell Adhesion Molecules |
| Zdroj: | JNCI Journal of the National Cancer Institute Journal of the National Cancer Institute, 101(12), 896-897. Oxford University Press Journal of the National Cancer Institute, 101(1), 61-66. Oxford University Press |
| ISSN: | 1460-2105 0027-8874 |
| Popis: | Identification of specific subtypes of circulating tumor cells in peripheral blood of cancer patients can provide information about the biology of metastasis and improve patient management. However, to be effective, the method used to identify circulating tumor cells must detect all tumor cell types. We investigated whether the five subtypes of human breast cancer cells that have been defined by global gene expression profiling-025EFnormal-like, basal, HER2-positive, and luminal A and B-025EFwere identified by CellSearch, a US Food and Drug Administration-approved test that uses antibodies against the cell surface-expressed epithelial cell adhesion molecule (EpCAM) to isolate circulating tumor cells. We used global gene expression profiling to determine the subtypes of a well-defined panel of 34 human breast cancer cell lines (15 luminal, nine normal-like, five basal-like, and five Her2-positive). We mixed 50-150 cells from 10 of these cell lines with 7.5 mL of blood from a single healthy human donor, and the mixtures were subjected to the CellSearch test to isolate the breast cancer cells. We found that the CellSearch isolation method, which uses EpCAM on the surface of circulating tumor cells for cell isolation, did not recognize, in particular, normal-like breast cancer cells, which in general have aggressive features. New tests that include antibodies that specifically recognize normal-like breast tumor cells but not cells of hematopoietic origin are needed. |
| Databáze: | OpenAIRE |
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