ATPase activity of SopA, a protein essential for active partitioning of F plasmid
| Autor: | Masaaki Wachi, Kazuo Nagai, Eijiro Watanabe, Makari Yamasaki |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
DNA Replication
DNA Bacterial ATPase Restriction Mapping medicine.disease_cause F Factor chemistry.chemical_compound Adenosine Triphosphate Plasmid Bacterial Proteins Escherichia coli Genetics medicine Molecular Biology Magnesium ion Adenosine Triphosphatases chemistry.chemical_classification biology Plasmid partitioning DNA replication Molecular biology Enzyme Biochemistry chemistry Genes Bacterial biology.protein DNA |
| Zdroj: | Molecular and General Genetics MGG. 234:346-352 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00538693 |
| Popis: | The sopA, B, C genes of the F plasmid play an essential role in plasmid partitioning during cell division in Escherichia coli. In this paper, the products of the sopA and sopB genes were isolated and their biochemical activities studied. [alpha-32P]ATP was cross-linked to the SopA protein by UV irradiation; this cross-linking was observed only in the presence of magnesium ion, and was competitively inhibited in the presence of non-radioactive ATP, ADP and dATP, but not other NTPs or dNTPs. In contrast, no ATP binding activity was detected for the SopB protein. The SopA protein showed a modest magnesium ion-dependent ATPase activity and this activity was stimulated in the presence of DNA. The ATPase activity in the presence of DNA was further stimulated by addition of the SopB protein. However, the SopB protein alone failed to stimulate the ATPase activity. |
| Databáze: | OpenAIRE |
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