Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch)
| Autor: | Mohammad Shahneawz Khan, Md. Monirul Islam, Mohammad Arif, Md. Kawser Ahmed, Mosammat Salma Akter, Elora Parvin |
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| Rok vydání: | 2011 |
| Předmět: |
Gills
animal structures Cell Survival DNA damage Lead chloride Fresh Water Anabas testudineus Management Monitoring Policy and Law Kidney medicine.disease_cause Toxicology medicine Animals General Environmental Science Gel electrophoresis Perch Dose-Response Relationship Drug biology General Medicine biology.organism_classification Pollution Molecular biology Comet assay Lead Liver Perches Toxicity Comet Assay Water Pollutants Chemical Genotoxicity DNA Damage Mutagens |
| Zdroj: | Environmental Monitoring and Assessment. 182:197-204 |
| ISSN: | 1573-2959 0167-6369 |
| DOI: | 10.1007/s10661-010-1869-6 |
| Popis: | The present investigation was undertaken to study the induction of DNA damage by lead chloride (PbCl(2)) in freshwater climbing perch Anabas testudineus using alkaline single cell gel electrophoresis (comet assay). Based on the LC(50) values of lead chloride of A. testudineus three different concentrations viz., 0.1, 1.0 and 2.0 mg/L were selected to expose fish. The DNA damage was observed in the gill, kidney and liver tissue as the percentage of DNA in comet tails and comet heads in the tissue of the exposed fish. DNA damage at different concentrations showed sensitivity to particular tissue. The liver tissue exhibited significantly (p0.01) higher DNA damage, followed by kidney and gill. However, the DNA damage was found to be dose dependent; at 2 mg/L of PbCl(2) the tail and head DNA of liver tissue were 57.84% and 39.49%, in kidney tissue the values were 52.36% and 44.97% whereas in gill tissue the values were 48.86% and 48.96% respectively. The current study explored the utility of the comet assay for in vivo laboratory studies using A. testudineus species for screening the genotoxic potential of lead chloride. |
| Databáze: | OpenAIRE |
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