CD34, CD117, and Actin Expression in Phyllodes Tumor of the Breast
| Autor: | Chun Liang Chang, Jean Shiunn Shyu, Cheng Jueng Chen, Horng-Jyh Harn, Chun Ming Chen, Huan Fa Hsieh |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Stromal cell Molecular Sequence Data Mammary gland CD34 Mitosis Antigens CD34 Breast Neoplasms macromolecular substances Polymerase Chain Reaction Proto-Oncogene Mas Antigen Antigens CD Phyllodes Tumor Gene expression otorhinolaryngologic diseases medicine Humans Amino Acid Sequence Aged Base Sequence biology CD117 Phyllodes tumor Middle Aged medicine.disease Actins carbohydrates (lipids) Proto-Oncogene Proteins c-kit stomatognathic diseases medicine.anatomical_structure biology.protein Cancer research Immunohistochemistry Female Surgery |
| Zdroj: | Journal of Surgical Research. 94:84-91 |
| ISSN: | 0022-4804 |
| DOI: | 10.1006/jsre.2000.6001 |
| Popis: | Background. This study investigated the immunophenotypic patterns of CD34, CD117 (a product of the c-kit proto-oncogene), and actin (HHF35) in benign and malignant phyllodes tumors (PTs). We correlated the expression of CD34, CD117, and actin with histopathological grade. Materials and results. We analyzed 19 cases (7 benign and 12 malignant cases) of PTs using immunohistochemical analysis. Six of 7 benign PT stromal lesions stained positively for CD34, while only 3 of 12 cases of malignant PT were focally CD34 positive ( P = 0.0106). Only 1 of the 7 benign PTs stromal lesions expressed CD117. Nine of the malignant PTs were composed CD117-positive fibroblasts. This result demonstrated that CD117 expression is associated with the malignant potential of PTs ( P = 0.0106). Actin (HHF-35) expression was found in 8 of 12 cases of malignant PTs ( P = 0.027), but in only 1 of 7 cases of benign PTs. Actin expression was significantly ( P = 0.04) correlated to frequent mitotic activity (>5 mitoses per 10 high-power fields). The immunophenotypic markers were not related to tumor size. Additionally, we sequenced part of the juxtamembrane region of the c-kit proto-oncogene and found point mutations in two malignant PTs. Conclusion. Our results demonstrated that expression of CD34 was associated with benign PTs, while CD117 and actin were preferentially expressed in malignant PTs. Our results implied that these immunohistological markers might be used for the histopathological grading of PTs. |
| Databáze: | OpenAIRE |
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