Control of cultured human cells with femtosecond laser ablated patterns on steel and plastic surfaces
| Autor: | Kimmo Paivasaari, Pasi Vahimaa, Martti Silvennoinen, Tarmo Nuutinen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Scanning electron microscope Surface Properties Biomedical Engineering Nanotechnology Cell Count 02 engineering and technology Cell Separation Grating 010402 general chemistry 01 natural sciences Article law.invention Hierarchical surface structures Molecular Imprinting law Cell Line Tumor Materials Testing Humans Cell behaviour Polycarbonate Molecular Biology Cells Cultured Cell Proliferation Cell Size Osteosarcoma Laser ablation Polycarboxylate Cement Cell growth Lasers 021001 nanoscience & nanotechnology Laser Human cells Stainless Steel 0104 chemical sciences visual_art Femtosecond visual_art.visual_art_medium Biophysics Elongation 0210 nano-technology |
| Zdroj: | Biomedical Microdevices |
| ISSN: | 1572-8781 1387-2176 |
| Popis: | The purpose of the present study is to explore topographical patterns produced with femtosecond laser pulses as a means of controlling the behaviour of living human cells (U2OS) on stainless steel surfaces and on negative plastic imprints (polycarbonate). The results show that the patterns on both types of material strongly affect cell behaviour and are particularly powerful in controlling cell spreading/elongation, localization and orientation. Analysis by fluorescence and scanning electron microscopy shows that on periodic 1D grating structures, cells and cell nuclei are highly elongated and aligned, whereas on periodic 2D grid structures, cell spreading and shape is affected. The results also show that the density and morphology of the cells can be affected. This was observed particularly on pseudo-periodic, coral-like structures which clearly inhibited cell growth. The results suggest that these patterns could be used in a variety of applications among the fields of clinical research and implant design, as well as in diagnosis and in cell and drug research. Furthermore, this article highlights the noteworthy aspects and the unique strengths of the technique and proposes directions for further research. Electronic supplementary material The online version of this article (doi:10.1007/s10544-012-9726-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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