Catalytic utilization of eIF-2 and mRNA binding proteins are limiting in lysates from vesicular stomatitis virus infected L cells
| Autor: | Ella Dratewka-Kos, Albert J. Wahba, Charles L. Woodley, Harshvardhan B. Mehta, Jean Lucas-Lenard, Ibolya Kiss |
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| Rok vydání: | 1984 |
| Předmět: |
Eukaryotic Initiation Factor-2
macromolecular substances environment and public health Biochemistry Vesicular stomatitis Indiana virus Mice Eukaryotic initiation factor 4F L Cells Methionine Reticulocyte Peptide Initiation Factors Eukaryotic initiation factor medicine Protein biosynthesis Animals Initiation factor RNA Messenger Eukaryotic Initiation Factors biology Binding protein Proteins biology.organism_classification Molecular biology Kinetics enzymes and coenzymes (carbohydrates) medicine.anatomical_structure Eukaryotic Initiation Factor-4F Vesicular stomatitis virus Protein Biosynthesis RNA Viral Ribosomes |
| Zdroj: | Biochemistry. 23:6184-6190 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00320a045 |
| Popis: | Infection of mouse L cells by vesicular stomatitis virus results in the inhibition of cellular protein synthesis. Lysates prepared from these infected cells are impaired in their ability to translate endogenous or exogenous cellular and viral mRNAs. The ability of initiation factors from rabbit reticulocytes to stimulate protein synthesis in these lysates was examined. Preparations of eukaryotic initiation factor 2 (eIF-2) and the guanine nucleotide exchange factor (GEF) stimulated protein synthesis strongly in L cell lysates from infected cells but only slightly in lysates from mock-infected cells. Maximal stimulation was obtained when a fraction containing eukaryotic initiation factors 4B (eIF-4B) and 4F (eIF-4F) was also present. In lysates from infected cells, these initiation factors increased endogenous cellular mRNA translation on the average 2-fold. In contrast, endogenous viral mRNA translation was increased to a much greater extent: the M protein was stimulated 8-fold, NS 5-fold, N 2.5-fold, and G 12-fold. When fractions containing eIF-4B, eIF-4F, or eIF-4A were added to these lysates in the presence of eIF-2, all three stimulated translation. Fractions containing rabbit reticulocyte initiation factors eIF-3 and eIF-6 had no effect on translation in either lysate. The results suggest that lysates from infected L cells are defective in the catalytic utilization of eIF-2 and deficient in mRNA binding protein activity. |
| Databáze: | OpenAIRE |
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