Recombination adenovirus-mediated human lactoferrin cDNA inhibits the growth of human MCF-7 breast cancer cells
| Autor: | Kun Li, Jianjie Wang, Shaobo Zhou, Qingwang Li, Zengsheng Han, Yetao Ou, Peijun Wang |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Complementary Blotting Western Genetic Vectors Green Fluorescent Proteins Pharmaceutical Science Apoptosis Breast Neoplasms medicine.disease_cause Polymerase Chain Reaction Adenoviridae Bcl-2-associated X protein Annexin Proto-Oncogene Proteins Tumor Cells Cultured medicine Humans bcl-2-Associated X Protein Pharmacology Bcl-2-Like Protein 11 biology Caspase 3 Lactoferrin Cell Cycle Membrane Proteins Cancer Genetic Therapy Cell cycle Flow Cytometry medicine.disease Molecular biology Gene Expression Regulation Neoplastic MCF-7 biology.protein Female Apoptosis Regulatory Proteins |
| Zdroj: | Journal of Pharmacy and Pharmacology. 64:457-463 |
| ISSN: | 2042-7158 0022-3573 |
| DOI: | 10.1111/j.2042-7158.2011.01410.x |
| Popis: | Objectives Human lactoferrin, an 80 kDa iron-binding glycoprotein, has antitumour effects. We have explored the potential therapeutic role of re-expressing human lactoferrin gene product in human breast cancer. Methods A recombinant adenovirus expressing the human lactoferrin cDNA (ad-hLTF) was constructed and used to infect breast cancer cells. Key findings Seventy-two hours after infection, ad-hLTF had considerable cytotoxicity on MCF-7 cells. A time-course study showed that ad-hLTF infection of MCF-7 cells at 100 plaque-forming units per cell increased the number of cells in G0/G1 phase and appeared markedly at Sub-G1 apoptotic peak. The presence of apoptotic cells was confirmed using Annexin V-fluoresecein isothiocyanate apoptosis detection by flow cytometry. Ad-hLTF also resulted in a decrease of Bcl-2 protein and an increase in Bax protein. Conclusions Ad-hLTF plays an important role in the induction of cell cycle arrest and apoptosis in MCF-7 cells. The results demonstrated that ad-hLTF could have potential benefits in the treatment of breast cancer. |
| Databáze: | OpenAIRE |
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