Activation of Macrophages and Cytotoxic Cells during Cytomegalovirus Pneumonia Complicating Lung Transplantations
| Autor: | Pierre Galanaud, Pierre Duroux, Jacques Cerrina, Dominique Emilie, Marc Humbert, Gérald Simonneau, O Devergne, Philippe Dartevelle, Bernadette Rain |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Adolescent Heart-Lung Transplantation Pneumonia Viral Congenital cytomegalovirus infection Gene Expression Lymphocyte Activation Granzymes Immune system Betaherpesvirinae medicine Humans Cytotoxic T cell Child Lung medicine.diagnostic_test biology Interleukin-6 Serine Endopeptidases Respiratory disease Macrophage Activation Middle Aged medicine.disease biology.organism_classification respiratory tract diseases Pneumonia Bronchoalveolar lavage medicine.anatomical_structure Cytomegalovirus Infections Immunology Female Interleukin-1 Lung Transplantation |
| Zdroj: | American Review of Respiratory Disease. 145:1178-1184 |
| ISSN: | 0003-0805 |
| Popis: | The functional status of immune cells within human transplanted lungs was analyzed during cytomegalovirus (CMV) pneumonia complicating lung and heart-lung transplantations. The expression of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) genes is a marker for the activation of macrophages as is that of serine esterase B (SE-B) gene for cytotoxic cells. The levels of expression of these genes by bronchoalveolar lavage (BAL) cells were determined by in situ hybridization. Eight cases of CMV pneumonia were included in this study. BAL cells from either rejection episodes (eight cases) or control transplanted patients experiencing neither infection nor allograft rejection (eight cases) were analyzed in parallel. In the control patients, virtually no cells expressed the IL-1 beta, the IL-6, or the SE-B genes. In contrast, these three genes were all expressed in samples from patients with CMV pneumonia. IL-1 beta gene-expressing cells were abundant in all infected patients (mean +/- SEM: 898 +/- 449 positive cells per 10(4) cells, p less than 0.001, compared with those in control patients). IL-6 gene-expressing cells were less numerous (92 +/- 74 positive cells per 10(4) cells) and present in five of the eight cases of CMV pneumonia. Activated cytotoxic cells were detected in seven of the eight cases of CMV pneumonia (36.5 +/- 19 SE-B gene-expressing cells per 10(4) cells, p less than 0.001). During allograft rejections (eight cases) IL-1 beta gene-expressing cells were present in all but one patient.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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