Combined Transplantation of Mesenchymal Stem Cells and Endothelial Colony-Forming Cells Accelerates Refractory Diabetic Foot Ulcer Healing
| Autor: | Xinxing Wan, Qian Xiao, Ke Chen, Honghui He, Liling Zhao, Pingyu Zeng, Zhaohui Mo, Yufang Luo, Wenjun Yang, Zi Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Article Subject Combination therapy 030204 cardiovascular system & hematology Umbilical cord 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Refractory Hyaluronic acid medicine Molecular Biology Internal medicine integumentary system business.industry Mesenchymal stem cell Cell Biology medicine.disease Diabetic foot RC31-1245 Surgery Transplantation 030104 developmental biology Diabetic foot ulcer medicine.anatomical_structure chemistry business Research Article |
| Zdroj: | Stem Cells International, Vol 2020 (2020) Stem Cells International |
| ISSN: | 1687-9678 |
| Popis: | Background. This study is aimed at investigating the effect of combined transplantation of umbilical cord mesenchymal stem cells (UCMSCs) and umbilical cord blood-derived endothelial colony-forming cells (ECFCs) on diabetic foot ulcer healing and at providing a novel therapy for chronic diabetic foot ulcer. Methods. We reported the treatment of refractory diabetic foot ulcers in twelve patients. Among them, five patients had two or more wounds; thus, one wound in the same patient was treated with cell injection, and other wounds were regarded as self-controls. The remaining seven patients had only one wound; therefore, the difference between the area of wound before and after treatment was estimated. The UCMSCs and ECFCs were injected into the wound along with topically applied hyaluronic acid (HA). Results. In this report, we compared the healing rate of multiple separate wounds in the same foot of the same patient: one treated with cell injection combined with topically applied HA-based hydrogel and was later covered by the hydrocolloid dressings, while the self-control wounds were only treated with conventional therapy and covered by the hydrocolloid dressings. The wound underwent cell injection showed accelerated healing in comparison to control wound within the first week after treatment. In other diabetic patients with only one refractory wound, the healing rate after cell transplantation was significantly faster than that before injection. Two large wounds healed without needing skin grafts after combination therapy of cell injection and HA. After four weeks of combination treatment, wound closure was reached in six patients, and the wounds of the other six patients were significantly reduced in size. Conclusions. Our study suggests that the combination of UCMSCs, ECFCs, and HA can safely synergize the accelerated healing of refractory diabetic foot ulcers. |
| Databáze: | OpenAIRE |
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